Production of transglutaminase by Streptomyces isolates in solid-state fermentation

V. Nagy, G. Szakács

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aims: To screen Streptomyces isolates for transglutaminase (TGase) production in solid-state fermentation (SSF) on various substrates. Methods and Results: Streptomyces mobaraensis NRRL B-3729, Streptomyces paucisporogenes ATCC 12596 and Streptomyces platensis NRRL 2364 strains were screened for extracellular TGase production in SSF on different substrates. High-protein-content beans, peas and lentils proved to be the best substrates. Good TGase production was obtained on liver kidney beans and green mung beans in a 4- to 6-day SSF. Temperature optima of the enzymes varied between 45 to 50°C. Molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) indicated similar size (∼37 kDa) for all three enzymes. TGase was the dominating protein band on SDS PAGE for two Streptomyces strains in SSF extracts. Other enzymes were present in smaller quantities. Conclusions: Streptomyces mobaraensis NRRL B-3729, S. paucisporogenes ATCC 12596 and S. platensis NRRL 2364 strains were successfully propagated under SSF conditions on crushed/milled liver kidney bean and green mung bean to obtain good level of TGase. Significance and Impact of the Study: Owing to much reduced production cost and direct applicability, SSF TGase without downstream processing (cheap in situ enzyme, crude enzyme) may be an excellent candidate for some nonfood applications.

Original languageEnglish
Pages (from-to)122-127
Number of pages6
JournalLetters in Applied Microbiology
Volume47
Issue number2
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Transglutaminases
Streptomyces
Fermentation
fermentation
Enzymes
enzyme
Phaseolus
Sodium dodecyl sulfate
Polyacrylates
Electrophoresis
substrate
Sodium Dodecyl Sulfate
Liver
Polyacrylamide Gel Electrophoresis
electrokinesis
Substrates
Gels
gel
In situ processing
sodium

Keywords

  • Beans
  • Hydroxamate
  • Solid-state fermentation
  • Streptomyces
  • Transglutaminase
  • Z-Gln-Gly

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

Cite this

Production of transglutaminase by Streptomyces isolates in solid-state fermentation. / Nagy, V.; Szakács, G.

In: Letters in Applied Microbiology, Vol. 47, No. 2, 08.2008, p. 122-127.

Research output: Contribution to journalArticle

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