Production of transgenic chimeric rabbits and transmission of the transgene through the germline

Szilárd Bodó, Elen Gócza, Tamás Révay, László Hiripi, Bogdan Carstea, A. Kovács, Lilla Bodrogi, Z. Bösze

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Here we report that improved reproductive technologies combined with an efficient microinjection method and in vitro cultivation medium enabled us to create germ line chimeric rabbits. To follow the fate of the chimeric embryo a blastomere marked with the human blood coagulation factor VIII (hFVIII) transgene was microinjected into a morula stage wild type embryo. The degree of chimerism in different tissues was estimated by real-time PCR and was found to be in the range of 0.1-42%. Among the four chimeric animals, one was identified as a chromosomal intersex and two were germline chimeras.

Original languageEnglish
Pages (from-to)435-440
Number of pages6
JournalMolecular Reproduction and Development
Volume68
Issue number4
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Transgenes
Embryonic Structures
Rabbits
Reproductive Techniques
Morula
Blastomeres
Chimerism
Factor VIII
Microinjections
Germ Cells
Real-Time Polymerase Chain Reaction
In Vitro Techniques

Keywords

  • Developmental biology
  • Germline chimeric rabbit
  • Real-time PCR
  • Reproductive technologies

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Production of transgenic chimeric rabbits and transmission of the transgene through the germline. / Bodó, Szilárd; Gócza, Elen; Révay, Tamás; Hiripi, László; Carstea, Bogdan; Kovács, A.; Bodrogi, Lilla; Bösze, Z.

In: Molecular Reproduction and Development, Vol. 68, No. 4, 08.2004, p. 435-440.

Research output: Contribution to journalArticle

Bodó, Szilárd ; Gócza, Elen ; Révay, Tamás ; Hiripi, László ; Carstea, Bogdan ; Kovács, A. ; Bodrogi, Lilla ; Bösze, Z. / Production of transgenic chimeric rabbits and transmission of the transgene through the germline. In: Molecular Reproduction and Development. 2004 ; Vol. 68, No. 4. pp. 435-440.
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