Production of transgenic chimeric rabbits and transmission of the transgene through the germline

Szilárd Bodó, Elen Gócza, Tamás Révay, László Hiripi, Bogdan Carstea, András Kovács, Lilla Bodrogi, Zsuzsanna Bösze

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Here we report that improved reproductive technologies combined with an efficient microinjection method and in vitro cultivation medium enabled us to create germ line chimeric rabbits. To follow the fate of the chimeric embryo a blastomere marked with the human blood coagulation factor VIII (hFVIII) transgene was microinjected into a morula stage wild type embryo. The degree of chimerism in different tissues was estimated by real-time PCR and was found to be in the range of 0.1-42%. Among the four chimeric animals, one was identified as a chromosomal intersex and two were germline chimeras.

Original languageEnglish
Pages (from-to)435-440
Number of pages6
JournalMolecular Reproduction and Development
Volume68
Issue number4
DOIs
Publication statusPublished - Aug 1 2004

Keywords

  • Developmental biology
  • Germline chimeric rabbit
  • Real-time PCR
  • Reproductive technologies

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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