Production of phosphinothricin-tolerant rice (Oryza sativa L.) through the application of phosphinothricin as growth regulator

O. Toldi, S. Tóth, A. S. Oreifig, E. Kiss, B. Jenes

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differentiation of 6-30 microshoots on 5-40% of the treated plant material was observed on a hormone-free culture medium supplemented with a sublethal concentration of PPT. We proved that PPT is morphogenetically active, similar to the action of many other herbicides, showing cytokinin-like effects in rice tissue culture. Fertile plants were grown from those microshoots having PPT tolerance under greenhouse conditions. To the best of our knowledge, this is the first report on the production of rice plants tolerant to this herbicide without genetic transformation. Since PPT is a competitive inhibitor of glutamine synthetase (GS), total GS activity in PPT-tolerant and PPT-sensitive plants was examined comprehensively in order to decide whether this enzyme has any role in PPT tolerance. An elevated GS activity was detected in PPT-tolerant plant material which could result in an elevated PPT tolerance at unchanged concentrations of the herbicide.

Original languageEnglish
Pages (from-to)1226-1231
Number of pages6
JournalPlant Cell Reports
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 1 2000

Keywords

  • Glutamine synthetase
  • Herbicide resistance
  • In vitro selection
  • Phosphinothricin
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Production of phosphinothricin-tolerant rice (Oryza sativa L.) through the application of phosphinothricin as growth regulator'. Together they form a unique fingerprint.

  • Cite this