Production of live offspring from vitrified-warmed oocytes collected at metaphase i stage

Ching Chien Chang, Wei Fang Chang, Jie Xu, An Sheng Cheng, Chia Chun Chang, P. Nagy, Cho Chen Yang, Shih Torng Ding, Li Ying Sung Sung

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Abstract

Vitrification of matured oocytes is widely adopted in human clinics and animal research laboratories. Cryopreservation of immature oocytes, particularly those at metaphase I (MI), remains a challenge. In the present work, mouse MI oocytes denuded of cumulus cells were vitrified and warmed (V/W) either prior to (V/W-BEFORE-IVM, n = 562) or after (V/W-AFTER-IVM, n = 664) in vitro maturation (IVM). Derivative metaphase II (MII) oocytes were then used for intracytoplasmic sperm injection (ICSI). In the control groups, in vivo matured MII oocytes were used freshly (FRESH-MII, n = 517) or after V/W (MII-V/W, n = 617). In vitro and in vivo developmental competencies were compared among groups. Satisfactory blastocyst rates were achieved in V/W-BEFORE-IVM (27.5%) and V/W-AFTER-IVM (32.4%) groups, albeit as expected still lower than those from fresh-MII (56.1%) or MII-V/W (45.6%) oocytes. Similarly, the term development rates from V/W-BEFORE-IVM and V/W-AFTER-IVM were 12.4% and 16.7% respectively, acceptable but lower than those of the fresh-MII (41.2%) and MII-V/W (23.3%) groups. These data demonstrate that oocytes collected at MI stage are amenable to V/W, which can be performed before or after IVM with acceptable development rates including production of healthy pups. These findings provide useful knowledge to researchers and clinical practitioners for preservation and use of the otherwise discarded MI oocytes.

Original languageEnglish
Article numbere0157785
JournalPLoS One
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chang, C. C., Chang, W. F., Xu, J., Cheng, A. S., Chang, C. C., Nagy, P., Yang, C. C., Ding, S. T., & Sung, L. Y. S. (2016). Production of live offspring from vitrified-warmed oocytes collected at metaphase i stage. PLoS One, 11(6), [e0157785]. https://doi.org/10.1371/journal.pone.0157785