Production of glucagon antibodies by thyroglobulin-zinc glucagon conjugate

L. Koranyi, F. Peterfy, A. Paksy, P. Vargha

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Of the 10 rabbits immunized with glucagon thyroglobulin (G-Tg) conjugates, only one developed glucagon antibodies. In contrast, all the surviving 8 rabbits in the ZnG-Tg (live glucagon thyroglobulin) group produced high-titer glucagon antiserum following the fifth immunization. After the tenth immunization the titers of the sera varied between 1:1000-1:30,000/titer is defined as the final dilution of the serum capable of binding 50% of the tracer added. Two of the sera proved to be specific for pancreatic glucagon, and crossreactivity with enteroglucagon was less than 1%. The observed difference between two immunogens is due to zinc ions which prolong the glucagon absorption and its conjugation characteristics. Probably zinc ions protect the C terminal portions of glucagon, whereby thyroglobulin is coupled mainly to its N terminal portions. At the time of absorption C terminal portions are free again to generate C terminal antibodies specific for pancreatic glucagon, as opposed to N terminal antibodies binding both pancreatic and enteroglucagon.

Original languageEnglish
Pages (from-to)434-435
Number of pages2
JournalHormone and Metabolic Research
Volume9
Issue number5
Publication statusPublished - 1977

Fingerprint

Thyroglobulin
Glucagon
Antibody Formation
Zinc
Antibodies
Glucagon-Like Peptides
Immunization
Serum
Ions
Rabbits
Dilution
Immune Sera

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Production of glucagon antibodies by thyroglobulin-zinc glucagon conjugate. / Koranyi, L.; Peterfy, F.; Paksy, A.; Vargha, P.

In: Hormone and Metabolic Research, Vol. 9, No. 5, 1977, p. 434-435.

Research output: Contribution to journalArticle

Koranyi, L. ; Peterfy, F. ; Paksy, A. ; Vargha, P. / Production of glucagon antibodies by thyroglobulin-zinc glucagon conjugate. In: Hormone and Metabolic Research. 1977 ; Vol. 9, No. 5. pp. 434-435.
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