Production of cellullolytic enzymes by a newly isolated, Trichoderma sp. FETL c3-2 via solid state fermentation grown on sugar cane baggase

Palm kernel cake as substrates

Pang Pei Kheng, Darah Ibrahim, L. Poppe, George Szackacs, Ibrahim Che Omar

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The production of cellulase by a local isolate Trichoderma sp. FETL c3-2 via solid state fermentation system using sugar cane baggase:palm kernel cake as substrate mixture was investigated. The optimized Solid State Fermentation (SSF) system consists of 5 g of sugar cane baggase: palm kernel cake, moisture content of 75% (w/w), pH of moistening agent of pH 7.0, at 30°C and inoculum size of 1×108 spores mL-1. The SSF system was also supplemented with 4% (w/w) dextrin and 6% (w/w) of yeast extract as additional carbon and nitrogen sources, respectively. Cellulose at the concentration of 0.2% (w/w) was found to be a significant inducer for cellulase production. Using the optimized SSF system, maximum FPase and CMCase production of 3.3 U g -1 substrate and 18.05 U g-1 substrate, respectively, after 4 days of fermentation time at 30°C were obtained. The modification of the SSF system resulted a 105% increment of FPase and 63% of CMCase production. Although no significant increase in growth after modification was observed, the results obtained indicated that the cellulase production by Trichoderma sp. FETL c3-2 is growth dependent.

Original languageEnglish
Pages (from-to)1430-1437
Number of pages8
JournalPakistan Journal of Biological Sciences
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 2006

Fingerprint

palm kernel cake
solid state fermentation
Trichoderma
sugarcane
endo-1,4-beta-glucanase
enzymes
dextrins
yeast extract
inoculum
cellulose
spores
fermentation
water content
carbon
nitrogen

Keywords

  • Cellulolytic enzymes
  • Palm kernel cake
  • Solid state fermentation
  • Sugar cane baggase
  • Trichoderma sp. FETL c3-2

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Production of cellullolytic enzymes by a newly isolated, Trichoderma sp. FETL c3-2 via solid state fermentation grown on sugar cane baggase : Palm kernel cake as substrates. / Kheng, Pang Pei; Ibrahim, Darah; Poppe, L.; Szackacs, George; Omar, Ibrahim Che.

In: Pakistan Journal of Biological Sciences, Vol. 9, No. 8, 08.2006, p. 1430-1437.

Research output: Contribution to journalArticle

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abstract = "The production of cellulase by a local isolate Trichoderma sp. FETL c3-2 via solid state fermentation system using sugar cane baggase:palm kernel cake as substrate mixture was investigated. The optimized Solid State Fermentation (SSF) system consists of 5 g of sugar cane baggase: palm kernel cake, moisture content of 75{\%} (w/w), pH of moistening agent of pH 7.0, at 30°C and inoculum size of 1×108 spores mL-1. The SSF system was also supplemented with 4{\%} (w/w) dextrin and 6{\%} (w/w) of yeast extract as additional carbon and nitrogen sources, respectively. Cellulose at the concentration of 0.2{\%} (w/w) was found to be a significant inducer for cellulase production. Using the optimized SSF system, maximum FPase and CMCase production of 3.3 U g -1 substrate and 18.05 U g-1 substrate, respectively, after 4 days of fermentation time at 30°C were obtained. The modification of the SSF system resulted a 105{\%} increment of FPase and 63{\%} of CMCase production. Although no significant increase in growth after modification was observed, the results obtained indicated that the cellulase production by Trichoderma sp. FETL c3-2 is growth dependent.",
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