Production of bacteriolytic enzymes by mycoparasitic Trichoderma strains

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Abstract

Eighteen mycoparasitic Trichoderma strains were tested for their ability to degrade heat-inactivated Bacillus cereus var. mycoides, B. megaterium, B. subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa and Serratia marcescens cells. The non-inductive and inductive ferment broths of five strains with good degrading abilities towards B. subtilis were investigated for specific degrading enzyme activities. In addition to trypsin- and chymotrypsin-like protease activities, β-1,4-N-acetyl-glucosaminidase (NAGase) was also secreted. Strain Trichoderma harzianum T19 had the most outstanding degrading abilities. The extracellular degrading enzymes of this strain were separated on a Sephadex G-150 column, and their preliminary characterization was performed. The results demonstrated that muramidase-like activities are present in the ferment broth of this T. harzianum strain.

Original languageEnglish
Pages (from-to)147-150
Number of pages4
JournalWorld Journal of Microbiology and Biotechnology
Volume18
Issue number2
DOIs
Publication statusPublished - Mar 26 2002

Keywords

  • Bacteria
  • Biocontrol
  • Extracellular enzymes
  • Gel filtration chromatography
  • Muramidase
  • Trichoderma

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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