Production of β-glucosidase in mixed culture of Aspergillus niger BKMF 1305 and Trichoderma reesei RUT C30

Tamás Juhász, Krisztina Kozma, Zs. Szengyel, K. Réczey

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The aim of the present study was to investigate a new approach to β-glucosidase production of an Aspergillus strain using cheap lignocellulosic material i.e. waste paper in order to substitute glucose, a generally used carbon source, and thereby reduce the production cost. The enzyme production was performed and optimized for the highest β-glucosidase yield in cofermentation with a Trichoderma strain to support the degradation of cellulose and to provide the non-cellulolytic Aspergillus with water soluble carbon source. Batch fermentation experiments of Aspergillus niger BKMF 1305 and Trichoderma reesei RUT C30 were carried out in shake flask cultures. The factors influencing the enzyme production, such as the concentrations of nutrients and carbon source, the inoculum ratio of the two species, and the delay in A. niger inoculation were investigated using a 23 full factorial design. The results were analyzed with the response surface methodology using commercially available software, Statistica for Windows. All three examined factors were found significant. The highest β-glucosidase activity of 3.07 IU/mL was obtained after 7 days of incubation, if 3.3% Aspergillus and 6.7 % Trichoderma inoculum were added at the same time to modified Mandels' medium, in which the concentration of nutrients was doubled compared to normal Mandels' medium and the carbon source concentration was set to 20 g/L waste paper.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalFood Technology and Biotechnology
Volume41
Issue number1
Publication statusPublished - Jan 2003

Fingerprint

Glucosidases
Trichoderma reesei
glucosidases
Trichoderma
Aspergillus niger
Aspergillus
mixed culture
Carbon
waste paper
Waste paper
carbon
Nutrients
inoculum
nutrient content
Enzymes
Food
batch fermentation
Batch Cell Culture Techniques
enzymes
response surface methodology

Keywords

  • β-glucosidase production
  • Aspergillus niger
  • Cofermentation
  • Trichoderma reesei

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Production of β-glucosidase in mixed culture of Aspergillus niger BKMF 1305 and Trichoderma reesei RUT C30. / Juhász, Tamás; Kozma, Krisztina; Szengyel, Zs.; Réczey, K.

In: Food Technology and Biotechnology, Vol. 41, No. 1, 01.2003, p. 49-53.

Research output: Contribution to journalArticle

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