Production and purification of low calcium response protein H of Chlamydophila pneumoniae

Ildikó Faludi, Ágnes Csanádi, Ágnes Míra Szabó, K. Burián, V. Endrész, A. Miczák

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the middle to late stages of the chlamydial developmental cycle. Gene of LcrH (CPn0811) in a 6His-tagged form was cloned from C. pneumoniae CWL029, expressed and purified from Escherichia coli using the HIS-select TALON CellThru Resin. The purity was checked with mass spectrometry. The samples were used for immunization of BALB/c mice. The inducible E. coli clone, which over-expresses the chlamydial LcrH, permits the study of the biological properties of this protein.

Original languageEnglish
Pages (from-to)389-397
Number of pages9
JournalActa Microbiologica et Immunologica Hungarica
Volume56
Issue number4
DOIs
Publication statusPublished - Dec 1 2009

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Chlamydophila pneumoniae
Calcium
Proteins
Escherichia coli
Cytosol
Immunization
Mass Spectrometry
Clone Cells
Bacteria
Membranes
Genes

Keywords

  • CPn0811
  • LcrH
  • Over-expression

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Production and purification of low calcium response protein H of Chlamydophila pneumoniae. / Faludi, Ildikó; Csanádi, Ágnes; Szabó, Ágnes Míra; Burián, K.; Endrész, V.; Miczák, A.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 56, No. 4, 01.12.2009, p. 389-397.

Research output: Contribution to journalArticle

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