Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation

Parameswaran Binod, T. Pusztahelyi, Viviana Nagy, Chandran Sandhya, G. Szakács, I. Pócsi, Ashok Pandey

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Fourteen Penicillium strains have been screened on wheat bran-crude chitin mixture medium for extracellular chitinase production in solid-state fermentation. Under the experimental conditions tested, Penicillium aculeatum NRRL 2129 (=ATCC 10409) was selected as the best enzyme producer. The optimum incubation period for chitinase production by the potent organism was found to be 72 h. Chromatofocusing was performed as the first step in the purification scheme, but high amount of contaminating proteins interfered with the method. Hence, ion-exchange chromatography experiments were carried out followed by gel filtration to separate and isolate chitinase isoenzymes. Four major chitinase peaks of molecular weight 82.7, 44.6, 28.2 and 26.9 kDa were observed after gel filtration chromatography while, on SDS-PAGE, three protein bands of molecular weights 82.6, 33.9 and 29.1 kDa were identified. The purified enzyme showed optimal temperature and pH at 50 and 5.5°C, respectively.

Original languageEnglish
Pages (from-to)880-887
Number of pages8
JournalEnzyme and Microbial Technology
Volume36
Issue number7
DOIs
Publication statusPublished - May 16 2005

Fingerprint

Chitinases
Penicillium
Chromatography
Fermentation
Purification
Gels
Enzymes
Molecular weight
Isoenzymes
Proteins
Chitin
Gel Chromatography
Ion exchange
Molecular Weight
Ion Exchange Chromatography
Dietary Fiber
Polyacrylamide Gel Electrophoresis
Experiments
Temperature

Keywords

  • Chitinase
  • Chromatography
  • Penicillium
  • Purification
  • Solid-state fermentation

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation. / Binod, Parameswaran; Pusztahelyi, T.; Nagy, Viviana; Sandhya, Chandran; Szakács, G.; Pócsi, I.; Pandey, Ashok.

In: Enzyme and Microbial Technology, Vol. 36, No. 7, 16.05.2005, p. 880-887.

Research output: Contribution to journalArticle

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