Production and meiotic pairing behaviour of new hybrids of winter wheat (Triticum aestivum) x winter barley (Hordeum vulgare)

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Abstract

New winter wheat (Triticum aestivum L.) x winter barley (Hordeum vulgare L.) hybrids produced using cultivated varieties (wheat 'Martonvásári 9 kr1'(Mv9 kr1) x barley 'Igri', Mv9 kr1 x 'Osnova', 'Asakazekomugi' x 'Manas') were multiplied in tissue culture because of the high degree of sterility and then pollinated with wheat to obtain backcross progenies. Meiotic analysis of the hybrids Mv9 kr1 x 'Igri' and 'Asakazekomugi' x 'Manas' and their in vitro regenerated progenies with the Feulgen method revealed 1.59 chromosome arm associations per cell in both initial hybrids. The number of chromosome arm associations increased after in vitro culture to 4.72 and 2.67, respectively, in the two combinations. According to the genomic in situ hybridization (GISH) analysis, wheat-barley chromosome arm associations made up 3.6% of the total in the initial Mv9 kr1 x 'Igri' hybrid and 6.6% and 16.5% of the total in vitro regenerated progenies of the 'Asakazekomugi' x 'Manas' and Mv9 kr1 x 'Igri' hybrids, respectively. The demonstration by GISH of wheat-barley chromosome pairing in the hybrids and especially in their in vitro regenerated progenies proves the possibility of producing recombinants between these two genera, and thus of transferring useful characters from barley into wheat. In vitro conditions caused an increase in chromosome arm association frequency in both combinations and in fertility in some regenerants.

Original languageEnglish
Pages (from-to)1045-1054
Number of pages10
JournalGenome
Volume43
Issue number6
DOIs
Publication statusPublished - 2000

Fingerprint

winter barley
Hordeum
Hordeum vulgare
Triticum
winter wheat
Triticum aestivum
wheat
barley
chromosomes
Chromosomes
in situ hybridization
In Situ Hybridization
genomics
chromosome pairing
Chromosome Pairing
in vitro culture
tissue culture
Infertility
Fertility
In Vitro Techniques

Keywords

  • Barley
  • GISH
  • Intergeneric hybridization
  • Meiotic chromosome pairing
  • Wheat

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

@article{8cc240fe029c4c7eb35b25c58405dc46,
title = "Production and meiotic pairing behaviour of new hybrids of winter wheat (Triticum aestivum) x winter barley (Hordeum vulgare)",
abstract = "New winter wheat (Triticum aestivum L.) x winter barley (Hordeum vulgare L.) hybrids produced using cultivated varieties (wheat 'Martonv{\'a}s{\'a}ri 9 kr1'(Mv9 kr1) x barley 'Igri', Mv9 kr1 x 'Osnova', 'Asakazekomugi' x 'Manas') were multiplied in tissue culture because of the high degree of sterility and then pollinated with wheat to obtain backcross progenies. Meiotic analysis of the hybrids Mv9 kr1 x 'Igri' and 'Asakazekomugi' x 'Manas' and their in vitro regenerated progenies with the Feulgen method revealed 1.59 chromosome arm associations per cell in both initial hybrids. The number of chromosome arm associations increased after in vitro culture to 4.72 and 2.67, respectively, in the two combinations. According to the genomic in situ hybridization (GISH) analysis, wheat-barley chromosome arm associations made up 3.6{\%} of the total in the initial Mv9 kr1 x 'Igri' hybrid and 6.6{\%} and 16.5{\%} of the total in vitro regenerated progenies of the 'Asakazekomugi' x 'Manas' and Mv9 kr1 x 'Igri' hybrids, respectively. The demonstration by GISH of wheat-barley chromosome pairing in the hybrids and especially in their in vitro regenerated progenies proves the possibility of producing recombinants between these two genera, and thus of transferring useful characters from barley into wheat. In vitro conditions caused an increase in chromosome arm association frequency in both combinations and in fertility in some regenerants.",
keywords = "Barley, GISH, Intergeneric hybridization, Meiotic chromosome pairing, Wheat",
author = "M. Moln{\'a}r-L{\'a}ng and G. Linc and A. Logojan and J. Sutka",
year = "2000",
doi = "10.1139/gen-43-6-1045",
language = "English",
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T1 - Production and meiotic pairing behaviour of new hybrids of winter wheat (Triticum aestivum) x winter barley (Hordeum vulgare)

AU - Molnár-Láng, M.

AU - Linc, G.

AU - Logojan, A.

AU - Sutka, J.

PY - 2000

Y1 - 2000

N2 - New winter wheat (Triticum aestivum L.) x winter barley (Hordeum vulgare L.) hybrids produced using cultivated varieties (wheat 'Martonvásári 9 kr1'(Mv9 kr1) x barley 'Igri', Mv9 kr1 x 'Osnova', 'Asakazekomugi' x 'Manas') were multiplied in tissue culture because of the high degree of sterility and then pollinated with wheat to obtain backcross progenies. Meiotic analysis of the hybrids Mv9 kr1 x 'Igri' and 'Asakazekomugi' x 'Manas' and their in vitro regenerated progenies with the Feulgen method revealed 1.59 chromosome arm associations per cell in both initial hybrids. The number of chromosome arm associations increased after in vitro culture to 4.72 and 2.67, respectively, in the two combinations. According to the genomic in situ hybridization (GISH) analysis, wheat-barley chromosome arm associations made up 3.6% of the total in the initial Mv9 kr1 x 'Igri' hybrid and 6.6% and 16.5% of the total in vitro regenerated progenies of the 'Asakazekomugi' x 'Manas' and Mv9 kr1 x 'Igri' hybrids, respectively. The demonstration by GISH of wheat-barley chromosome pairing in the hybrids and especially in their in vitro regenerated progenies proves the possibility of producing recombinants between these two genera, and thus of transferring useful characters from barley into wheat. In vitro conditions caused an increase in chromosome arm association frequency in both combinations and in fertility in some regenerants.

AB - New winter wheat (Triticum aestivum L.) x winter barley (Hordeum vulgare L.) hybrids produced using cultivated varieties (wheat 'Martonvásári 9 kr1'(Mv9 kr1) x barley 'Igri', Mv9 kr1 x 'Osnova', 'Asakazekomugi' x 'Manas') were multiplied in tissue culture because of the high degree of sterility and then pollinated with wheat to obtain backcross progenies. Meiotic analysis of the hybrids Mv9 kr1 x 'Igri' and 'Asakazekomugi' x 'Manas' and their in vitro regenerated progenies with the Feulgen method revealed 1.59 chromosome arm associations per cell in both initial hybrids. The number of chromosome arm associations increased after in vitro culture to 4.72 and 2.67, respectively, in the two combinations. According to the genomic in situ hybridization (GISH) analysis, wheat-barley chromosome arm associations made up 3.6% of the total in the initial Mv9 kr1 x 'Igri' hybrid and 6.6% and 16.5% of the total in vitro regenerated progenies of the 'Asakazekomugi' x 'Manas' and Mv9 kr1 x 'Igri' hybrids, respectively. The demonstration by GISH of wheat-barley chromosome pairing in the hybrids and especially in their in vitro regenerated progenies proves the possibility of producing recombinants between these two genera, and thus of transferring useful characters from barley into wheat. In vitro conditions caused an increase in chromosome arm association frequency in both combinations and in fertility in some regenerants.

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KW - Intergeneric hybridization

KW - Meiotic chromosome pairing

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JO - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada

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