Production and hydrolytic efficiency of enzymes from Trichoderma reesei RUTC30 using steam pretreated wheat straw as carbon source

Pablo Alvira, Miklós Gyalai-Korpos, Zsolt Barta, José Miguel Oliva, K. Réczey, Mercedes Ballesteros

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Advances on enzyme technology have improved the economics of cellulosic ethanol. Nevertheless, there is still great interest to improve the production and the characteristics of the enzymatic preparations. One possibility to reduce the cost of the enzymes is on-site enzyme production in a biorefinery plant, in which part of the lignocellulosic material already available can be used as a cheap carbon source in the enzyme production. Results: Steam exploded wheat straw at two different severity conditions was evaluated as carbon source for enzyme production by Trichoderma reesei. The results showed that volumetric production of cellulases and β-glucosidase increased when using a carbon source with high cellulose content. When a carbon source with a higher content of hemicellulose was used the enzymatic mixture showed significantly higher proportions of hemicellulase activities (xylanase, β-xylosidase and α-L-arabinofuranosidase), which had a positive effect on subsequent enzymatic hydrolysis. Conclusions: The proportion of cellulose and hemicellulose in the carbon source has an influence on the composition of the enzymatic cocktail. The higher the cellulose and hemicellulose content, the higher the titer of cellulase and hemicellulase produced by the fungus, respectively. The enzyme preparations with higher hemicellulase activities were more efficient in hydrolyzing xylan-containing substrates.

Original languageEnglish
Pages (from-to)1150-1156
Number of pages7
JournalJournal of Chemical Technology and Biotechnology
Volume88
Issue number6
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Trichoderma
Steam
Straw
straw
Triticum
Carbon
Enzymes
wheat
enzyme
carbon
Cellulose
cellulose
Xylosidases
Cellulosic ethanol
Glucosidases
Cellulases
Xylans
Enzymatic hydrolysis
Cellulase
Fungi

Keywords

  • Cellulase production
  • Hemicellulase
  • Steam explosion
  • Trichoderma reesei
  • Wheat straw

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Renewable Energy, Sustainability and the Environment
  • Biotechnology
  • Chemical Engineering(all)
  • Fuel Technology

Cite this

Production and hydrolytic efficiency of enzymes from Trichoderma reesei RUTC30 using steam pretreated wheat straw as carbon source. / Alvira, Pablo; Gyalai-Korpos, Miklós; Barta, Zsolt; Oliva, José Miguel; Réczey, K.; Ballesteros, Mercedes.

In: Journal of Chemical Technology and Biotechnology, Vol. 88, No. 6, 06.2013, p. 1150-1156.

Research output: Contribution to journalArticle

Alvira, Pablo ; Gyalai-Korpos, Miklós ; Barta, Zsolt ; Oliva, José Miguel ; Réczey, K. ; Ballesteros, Mercedes. / Production and hydrolytic efficiency of enzymes from Trichoderma reesei RUTC30 using steam pretreated wheat straw as carbon source. In: Journal of Chemical Technology and Biotechnology. 2013 ; Vol. 88, No. 6. pp. 1150-1156.
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