Production and fish identification of wheat- Aegilops biuncialis addition lines and their use for the selection of U and M genome-specific molecular (SSR) markers

A. Schneider, I. Molnár, M. Molnár-Láng

Research output: Contribution to journalArticle

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Abstract

One way of incorporating useful traits from Aegilops biuncialis (2n=4x=28, U b U b M b M b) into wheat (Triticum aestivum L. 2n=6x=42, AABBDD) is to develop first addition then translocation lines. The 2M b , 3M b , 7M b , 3U b , 5U b and 5U b /6U b wheat- Ae. biuncialis addition lines were produced in Martonvásár. To facilitate the exact identification of the addition lines, it was necessary to analyse the fluorescence in situ hybridisation patterns of the parental wheat genotype, Ae. biuncialis and its diploid progenitors (Ae. umbellulata 2n=2x=14, UU and Ae. comosa 2n=2x=14, MM). The great genetic variability of the Aegilops species causes polymorphism in the fluorescence in situ hybridisation (FISH) patterns of the individual chromosomes. Due to the high level of FISH polymorphism, it is advisable to confirm the identification of the Ae. biuncialis chromosomes with the help of molecular (microsatellite, SSR) markers, so 119 wheat SSR markers were tested on Aegilops biuncialis , on Ae. geniculata (2n=4x=28, U g U g M g M g), on five wheat- Ae. biuncialis addition lines (2M b , 3M b , 7M b , 3U b , 5U b) and on an addition series of wheat- Ae. geniculata in order to select SSR markers specific to the U and M genomes of Ae. biuncialis and Ae. geniculata .

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalActa Agronomica Hungarica
Volume58
Issue number2
DOIs
Publication statusPublished - Jun 1 2010

Fingerprint

Aegilops biuncialis
wheat
genome
fish
fluorescence in situ hybridization
genetic polymorphism
translocation lines
chromosomes
Aegilops
diploidy
Triticum aestivum
microsatellite repeats
genetic variation

Keywords

  • Addition lines
  • Ae. biuncialis
  • Ae. geniculata
  • In situ hybridisation
  • SSR markers

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Production and fish identification of wheat- Aegilops biuncialis addition lines and their use for the selection of U and M genome-specific molecular (SSR) markers",
abstract = "One way of incorporating useful traits from Aegilops biuncialis (2n=4x=28, U b U b M b M b) into wheat (Triticum aestivum L. 2n=6x=42, AABBDD) is to develop first addition then translocation lines. The 2M b , 3M b , 7M b , 3U b , 5U b and 5U b /6U b wheat- Ae. biuncialis addition lines were produced in Martonv{\'a}s{\'a}r. To facilitate the exact identification of the addition lines, it was necessary to analyse the fluorescence in situ hybridisation patterns of the parental wheat genotype, Ae. biuncialis and its diploid progenitors (Ae. umbellulata 2n=2x=14, UU and Ae. comosa 2n=2x=14, MM). The great genetic variability of the Aegilops species causes polymorphism in the fluorescence in situ hybridisation (FISH) patterns of the individual chromosomes. Due to the high level of FISH polymorphism, it is advisable to confirm the identification of the Ae. biuncialis chromosomes with the help of molecular (microsatellite, SSR) markers, so 119 wheat SSR markers were tested on Aegilops biuncialis , on Ae. geniculata (2n=4x=28, U g U g M g M g), on five wheat- Ae. biuncialis addition lines (2M b , 3M b , 7M b , 3U b , 5U b) and on an addition series of wheat- Ae. geniculata in order to select SSR markers specific to the U and M genomes of Ae. biuncialis and Ae. geniculata .",
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T1 - Production and fish identification of wheat- Aegilops biuncialis addition lines and their use for the selection of U and M genome-specific molecular (SSR) markers

AU - Schneider, A.

AU - Molnár, I.

AU - Molnár-Láng, M.

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AB - One way of incorporating useful traits from Aegilops biuncialis (2n=4x=28, U b U b M b M b) into wheat (Triticum aestivum L. 2n=6x=42, AABBDD) is to develop first addition then translocation lines. The 2M b , 3M b , 7M b , 3U b , 5U b and 5U b /6U b wheat- Ae. biuncialis addition lines were produced in Martonvásár. To facilitate the exact identification of the addition lines, it was necessary to analyse the fluorescence in situ hybridisation patterns of the parental wheat genotype, Ae. biuncialis and its diploid progenitors (Ae. umbellulata 2n=2x=14, UU and Ae. comosa 2n=2x=14, MM). The great genetic variability of the Aegilops species causes polymorphism in the fluorescence in situ hybridisation (FISH) patterns of the individual chromosomes. Due to the high level of FISH polymorphism, it is advisable to confirm the identification of the Ae. biuncialis chromosomes with the help of molecular (microsatellite, SSR) markers, so 119 wheat SSR markers were tested on Aegilops biuncialis , on Ae. geniculata (2n=4x=28, U g U g M g M g), on five wheat- Ae. biuncialis addition lines (2M b , 3M b , 7M b , 3U b , 5U b) and on an addition series of wheat- Ae. geniculata in order to select SSR markers specific to the U and M genomes of Ae. biuncialis and Ae. geniculata .

KW - Addition lines

KW - Ae. biuncialis

KW - Ae. geniculata

KW - In situ hybridisation

KW - SSR markers

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U2 - 10.1556/AAgr.58.2010.2.6

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JO - Acta Agronomica Hungarica

JF - Acta Agronomica Hungarica

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