Production and fish identification of wheat- Aegilops biuncialis addition lines and their use for the selection of U and M genome-specific molecular (SSR) markers

A. Schneider, I. Molnár, M. Molnár-Láng

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Abstract

One way of incorporating useful traits from Aegilops biuncialis (2n=4x=28, U b U b M b M b) into wheat (Triticum aestivum L. 2n=6x=42, AABBDD) is to develop first addition then translocation lines. The 2M b , 3M b , 7M b , 3U b , 5U b and 5U b /6U b wheat- Ae. biuncialis addition lines were produced in Martonvásár. To facilitate the exact identification of the addition lines, it was necessary to analyse the fluorescence in situ hybridisation patterns of the parental wheat genotype, Ae. biuncialis and its diploid progenitors (Ae. umbellulata 2n=2x=14, UU and Ae. comosa 2n=2x=14, MM). The great genetic variability of the Aegilops species causes polymorphism in the fluorescence in situ hybridisation (FISH) patterns of the individual chromosomes. Due to the high level of FISH polymorphism, it is advisable to confirm the identification of the Ae. biuncialis chromosomes with the help of molecular (microsatellite, SSR) markers, so 119 wheat SSR markers were tested on Aegilops biuncialis , on Ae. geniculata (2n=4x=28, U g U g M g M g), on five wheat- Ae. biuncialis addition lines (2M b , 3M b , 7M b , 3U b , 5U b) and on an addition series of wheat- Ae. geniculata in order to select SSR markers specific to the U and M genomes of Ae. biuncialis and Ae. geniculata .

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalActa Agronomica Hungarica
Volume58
Issue number2
DOIs
Publication statusPublished - Jun 1 2010

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Keywords

  • Addition lines
  • Ae. biuncialis
  • Ae. geniculata
  • In situ hybridisation
  • SSR markers

ASJC Scopus subject areas

  • Agronomy and Crop Science

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