Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish

Saravanane Poobalane, Kim D. Thompson, László Ardó, Noel Verjan, Hyun Ja Han, Galina Jeney, Ikuo Hirono, Takashi Aoki, Alexandra Adams

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55 Citations (Scopus)


A recombinant protein for the S-layer protein of Aeromonas hydrophila was produced and its ability to protect common carp Cyprinus carpio L. against six virulent isolates of A. hydrophila was assessed. A group of 120 carp (30-40 g) were vaccinated intra-peritoneally with 0.1 ml of adjuvanted vaccine (30 μg protein per fish). Another group of 120 carp were injected with 0.1 ml of PBS-adjuvant mixture to serve as controls. Twenty fish from each group were challenged with each one of six virulent isolates of A. hydrophila 35 days post-vaccination. The fish were maintained in 12 separate tanks before terminating the experiment at 16 days post-challenge. The relative percentage survival (RPS) for the six isolates of A. hydrophila ranged from 56 to 87%. The difference in survival rate of fish challenged with four of the isolates was statistically significant in vaccinated fish compared to control fish, when analysed using a Chi-square test. The results of the study suggest that the recombinant S-layer protein of A. hydrophila could be useful as a vaccine antigen to protect fish against different isolates of this pathogenic bacterium.

Original languageEnglish
Pages (from-to)3540-3547
Number of pages8
Issue number20
Publication statusPublished - Apr 30 2010



  • Aeromonas hydrophila
  • Common carp
  • Efficacy testing
  • Recombinant S-layer protein vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Poobalane, S., Thompson, K. D., Ardó, L., Verjan, N., Han, H. J., Jeney, G., Hirono, I., Aoki, T., & Adams, A. (2010). Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish. Vaccine, 28(20), 3540-3547.