Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish

Saravanane Poobalane, Kim D. Thompson, László Ardó, Noel Verjan, Hyun Ja Han, G. Jeney, Ikuo Hirono, Takashi Aoki, Alexandra Adams

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A recombinant protein for the S-layer protein of Aeromonas hydrophila was produced and its ability to protect common carp Cyprinus carpio L. against six virulent isolates of A. hydrophila was assessed. A group of 120 carp (30-40 g) were vaccinated intra-peritoneally with 0.1 ml of adjuvanted vaccine (30 μg protein per fish). Another group of 120 carp were injected with 0.1 ml of PBS-adjuvant mixture to serve as controls. Twenty fish from each group were challenged with each one of six virulent isolates of A. hydrophila 35 days post-vaccination. The fish were maintained in 12 separate tanks before terminating the experiment at 16 days post-challenge. The relative percentage survival (RPS) for the six isolates of A. hydrophila ranged from 56 to 87%. The difference in survival rate of fish challenged with four of the isolates was statistically significant in vaccinated fish compared to control fish, when analysed using a Chi-square test. The results of the study suggest that the recombinant S-layer protein of A. hydrophila could be useful as a vaccine antigen to protect fish against different isolates of this pathogenic bacterium.

Original languageEnglish
Pages (from-to)3540-3547
Number of pages8
JournalVaccine
Volume28
Issue number20
DOIs
Publication statusPublished - Apr 30 2010

Fingerprint

Aeromonas hydrophila
Fishes
Vaccines
Carps
vaccines
fish
proteins
Cyprinus carpio
carp
Fish Proteins
Protein S
Staphylococcal Protein A
Chi-Square Distribution
Recombinant Proteins
S-layer proteins
recombinant proteins
Vaccination
adjuvants
Bacteria
Antigens

Keywords

  • Aeromonas hydrophila
  • Common carp
  • Efficacy testing
  • Recombinant S-layer protein vaccine

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Poobalane, S., Thompson, K. D., Ardó, L., Verjan, N., Han, H. J., Jeney, G., ... Adams, A. (2010). Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish. Vaccine, 28(20), 3540-3547. https://doi.org/10.1016/j.vaccine.2010.03.011

Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish. / Poobalane, Saravanane; Thompson, Kim D.; Ardó, László; Verjan, Noel; Han, Hyun Ja; Jeney, G.; Hirono, Ikuo; Aoki, Takashi; Adams, Alexandra.

In: Vaccine, Vol. 28, No. 20, 30.04.2010, p. 3540-3547.

Research output: Contribution to journalArticle

Poobalane, S, Thompson, KD, Ardó, L, Verjan, N, Han, HJ, Jeney, G, Hirono, I, Aoki, T & Adams, A 2010, 'Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish', Vaccine, vol. 28, no. 20, pp. 3540-3547. https://doi.org/10.1016/j.vaccine.2010.03.011
Poobalane, Saravanane ; Thompson, Kim D. ; Ardó, László ; Verjan, Noel ; Han, Hyun Ja ; Jeney, G. ; Hirono, Ikuo ; Aoki, Takashi ; Adams, Alexandra. / Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish. In: Vaccine. 2010 ; Vol. 28, No. 20. pp. 3540-3547.
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