Prod is a novel DNA-binding protein that binds to the 1.686 g/cm3 10 bp satellite repeat of Drosophila melanogaster

Tibor Török, Mátyás Gorjánácz, Peter J. Bryant, I. Kiss

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The proliferation disrupter (prod) gene of Drosophila melanogaster encodes a novel protein associated with centromeric chromosomal regions that is required for chromatin condensation and cell viability. We have examined the binding of the Prod protein to DNA in vitro. Co-immunoprecipitation experiments demonstrate that Prod is a DNA-binding protein that specifically recognizes the 10 bp AGAATAACAT satellite repeat of D.melanogaster. Footprinting experiments show that the protein interacts with a 5-8 bp target sequence in each 10 bp repeat and suggest that it can mediate condensation of this satellite into a superhelix. Gel retardation experiments indicate that Prod does not have a well defined DNA-binding domain and it binds the satellite in a co-operative manner, probably forming Prod multimers. Since Prod localizes to both heterochromatin and euchromatin in vivo, we discuss the possibility that the ability of pre-existing euchromatic proteins to bind DNA in a co-operative manner, might be a prerequisite of satellite compaction and satellite amplification, thereby providing a basic factor in heterochromatin evolution.

Original languageEnglish
Pages (from-to)3551-3557
Number of pages7
JournalNucleic Acids Research
Volume28
Issue number18
Publication statusPublished - Sep 15 2000

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DNA-Binding Proteins
Drosophila melanogaster
Heterochromatin
DNA
Euchromatin
Proteins
Immunoprecipitation
Chromatin
Cell Survival
Carrier Proteins
Gels
Genes

ASJC Scopus subject areas

  • Genetics

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Prod is a novel DNA-binding protein that binds to the 1.686 g/cm3 10 bp satellite repeat of Drosophila melanogaster. / Török, Tibor; Gorjánácz, Mátyás; Bryant, Peter J.; Kiss, I.

In: Nucleic Acids Research, Vol. 28, No. 18, 15.09.2000, p. 3551-3557.

Research output: Contribution to journalArticle

Török, Tibor ; Gorjánácz, Mátyás ; Bryant, Peter J. ; Kiss, I. / Prod is a novel DNA-binding protein that binds to the 1.686 g/cm3 10 bp satellite repeat of Drosophila melanogaster. In: Nucleic Acids Research. 2000 ; Vol. 28, No. 18. pp. 3551-3557.
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