Problems and possibilities of wheat-maize somatic hybridization

I. Göntér, B. Szarka, Á Lendvai, M. Molnár-Láng, S. Mórocz, D. Dudits

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Protoplast fusion was performed between mesophyll cells of wheat (Triticum aestivum L.) and albino maize (Zea mays L.) by polyethylene glycol (PEG) treatments. The parental protoplasts were not able to produce green plants without fusion. The hybrid calli have mixed cytoplasm and regenerated green, maize-like plants, six months after the PEG treatment. The plants were sterile although had both female and male flowers. The RAPD analysis (using total DNA, and three primer combinations) produced bands resembling the wheat profile. The cytological analysis revealed 56 chromosomes in the root tip and callus cells, but intact wheat chromosome was not observed. Genomic in situ hybridization using total wheat DNA as probe revealed the presence of wheat DNA droplets in the maize chromosomal background. The flow cytometrical analysis showed intermediate DNA concentration in hybrid nucleus. Other intermediate morphological traits of plants with hybrid origin were not found.

Original languageEnglish
Pages (from-to)11-12
Number of pages2
JournalActa Biologica Szegediensis
Volume46
Issue number3-4
Publication statusPublished - 2002

Fingerprint

somatic hybridization
Triticum
Zea mays
wheat
corn
DNA
Chromosomes
polyethylene glycol
Fusion reactions
Protoplasts
Bony Callus
callus
chromosomes
protoplast fusion
Mesophyll Cells
male flowers
female flowers
Viridiplantae
DNA Primers
albino

Keywords

  • Flow cytometry
  • Protoplast fusion
  • RAPD analysis
  • Somatic hybrid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Applied Microbiology and Biotechnology
  • Neuroscience(all)

Cite this

Problems and possibilities of wheat-maize somatic hybridization. / Göntér, I.; Szarka, B.; Lendvai, Á; Molnár-Láng, M.; Mórocz, S.; Dudits, D.

In: Acta Biologica Szegediensis, Vol. 46, No. 3-4, 2002, p. 11-12.

Research output: Contribution to journalArticle

Göntér, I, Szarka, B, Lendvai, Á, Molnár-Láng, M, Mórocz, S & Dudits, D 2002, 'Problems and possibilities of wheat-maize somatic hybridization', Acta Biologica Szegediensis, vol. 46, no. 3-4, pp. 11-12.
Göntér, I. ; Szarka, B. ; Lendvai, Á ; Molnár-Láng, M. ; Mórocz, S. ; Dudits, D. / Problems and possibilities of wheat-maize somatic hybridization. In: Acta Biologica Szegediensis. 2002 ; Vol. 46, No. 3-4. pp. 11-12.
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