Problem-solving Test: DNA manipulation with alkaline phosphatase and polynucleotide kinase

Research output: Contribution to journalArticle

Abstract

Terms to be familiar with before you start to solve the test: 3′- and 5′-ends of DNA fragments, plasmids, restriction endonucleases, agarose gel electrophoresis, linear and circular DNA, superhelical DNA, blunt and sticky ends, DNA ligase, nuclease, [γ-32P] ATP, and radioactive labeling.

Original languageEnglish
Pages (from-to)348-350
Number of pages3
JournalBiochemistry and Molecular Biology Education
Volume42
Issue number4
DOIs
Publication statusPublished - 2014

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Polynucleotide 5'-Hydroxyl-Kinase
DNA Ligases
Superhelical DNA
Circular DNA
Deoxyribonucleases
Agar Gel Electrophoresis
DNA Restriction Enzymes
Electrophoresis
Sepharose
Labeling
Alkaline Phosphatase
Plasmids
Adenosine Triphosphate
Gels
DNA

Keywords

  • Biotechnology education
  • Molecular biology
  • Nucleic acid enzymology
  • Problem-based learning

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

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