Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering

Abdallah Oukarroum, Saïd El Madidi, G. Schansker, Reto J. Strasser

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

The main objective of this study was to evaluate the effects of drought and re-watering on 10 varieties of barley (Hordeum vulgare L.) originating from Morocco. Five varieties obtained from the National Institute of Agricultural Research (INRA) of Morocco and five landraces (local varieties defined by high stress tolerance, high yield stability, an intermediate yield and low-input demand) collected at five localities in the south of Morocco were used in the present study. After 2 weeks of growth, drought stress was initiated by withholding water for 2 weeks followed by 1 week of re-watering. The polyphasic OJIP fluorescence transient was used to evaluate photosystem II (PSII) criteria at the end of the first week of drought stress (moderate drought), at the end of the second week (severe drought) and the end of the recovery phase. Drought and re-watering had little effect on the maximum quantum yield of primary photochemistry φPo(=FV/FM). The photosynthetic performance index (PI) is the product of an antenna, reaction center and electron transport dependent parameter. It revealed differences between varieties as a function of drought and re-watering. For the screening for drought stress tolerance, changes in the PI during a 2-week drought stress treatment were analysed and a new parameter was defined: the drought factor index (DFI) = log(PIweek 1/PIcontrol) + 2 log(PIweek 2/PIcontrol). The DFI of the tested varieties correlated with their drought tolerance. Another parameter that was analysed was the relative water content. It decreased during the drought stress treatment varying between 61% and 78.2% at the end of the drought period. During the subsequent recovery period, it increased in a species-dependent manner (65.1-94.1%). A third parameter studied were changes in the initial fluorescence rise. The fluorescence rise during the first 300 μs (L-band) can give information on the energetic connectivity between PSII units whereas changes in the rise during the first 2 ms (K-band) offer information on developing limitations on the donor side of PSII. Changes in respectively the L and K-bands of the fluorescence transients OJIP were shown to have predictive value with respect to the vitality of leaves and the tolerance of the varieties to drought stress.

Original languageEnglish
Pages (from-to)438-446
Number of pages9
JournalEnvironmental and Experimental Botany
Volume60
Issue number3
DOIs
Publication statusPublished - Jul 2007

Fingerprint

drought stress
barley
Hordeum vulgare
cultivar
chlorophyll a
fluorescence
water stress
drought
irrigation
chlorophyll
cultivars
Morocco
photosystem II
tolerance
drought tolerance
photochemistry
agricultural research
stress tolerance
antennae
landraces

Keywords

  • Drought factor index
  • Drought tolerance
  • Hordeum vulgare L.
  • Performance index
  • Polyphasic fluorescence transient (OJIP)
  • Recovery

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Environmental Science(all)

Cite this

Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. / Oukarroum, Abdallah; Madidi, Saïd El; Schansker, G.; Strasser, Reto J.

In: Environmental and Experimental Botany, Vol. 60, No. 3, 07.2007, p. 438-446.

Research output: Contribution to journalArticle

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abstract = "The main objective of this study was to evaluate the effects of drought and re-watering on 10 varieties of barley (Hordeum vulgare L.) originating from Morocco. Five varieties obtained from the National Institute of Agricultural Research (INRA) of Morocco and five landraces (local varieties defined by high stress tolerance, high yield stability, an intermediate yield and low-input demand) collected at five localities in the south of Morocco were used in the present study. After 2 weeks of growth, drought stress was initiated by withholding water for 2 weeks followed by 1 week of re-watering. The polyphasic OJIP fluorescence transient was used to evaluate photosystem II (PSII) criteria at the end of the first week of drought stress (moderate drought), at the end of the second week (severe drought) and the end of the recovery phase. Drought and re-watering had little effect on the maximum quantum yield of primary photochemistry φPo(=FV/FM). The photosynthetic performance index (PI) is the product of an antenna, reaction center and electron transport dependent parameter. It revealed differences between varieties as a function of drought and re-watering. For the screening for drought stress tolerance, changes in the PI during a 2-week drought stress treatment were analysed and a new parameter was defined: the drought factor index (DFI) = log(PIweek 1/PIcontrol) + 2 log(PIweek 2/PIcontrol). The DFI of the tested varieties correlated with their drought tolerance. Another parameter that was analysed was the relative water content. It decreased during the drought stress treatment varying between 61{\%} and 78.2{\%} at the end of the drought period. During the subsequent recovery period, it increased in a species-dependent manner (65.1-94.1{\%}). A third parameter studied were changes in the initial fluorescence rise. The fluorescence rise during the first 300 μs (L-band) can give information on the energetic connectivity between PSII units whereas changes in the rise during the first 2 ms (K-band) offer information on developing limitations on the donor side of PSII. Changes in respectively the L and K-bands of the fluorescence transients OJIP were shown to have predictive value with respect to the vitality of leaves and the tolerance of the varieties to drought stress.",
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