Probing the cavity of the slow inactivated conformation of Shaker potassium channels

Gyorgy Panyi, Carol Deutsch

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Slow inactivation involves a local rearrangement of the outer mouth of voltage-gated potassium channels, but nothing is known regarding rearrangements in the cavity between the activation gate and the selectivity filter. We now report that the cavity undergoes a conformational change in the slow-inactivated state. This change is manifest as altered accessibility of residues facing the aqueous cavity and as a marked decrease in the affinity of tetraethylammonium for its internal binding site. These findings have implications for global alterations of the channel during slow inactivation and putative coupling between activation and slow-inactivation gates.

Original languageEnglish
Pages (from-to)403-418
Number of pages16
JournalJournal of General Physiology
Volume129
Issue number5
DOIs
Publication statusPublished - May 2007

ASJC Scopus subject areas

  • Physiology

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