Probing the activity of matrix metalloproteinase II with a sequentially click-labeled silica nanoparticle FRET probe

Daniela E. Achatz, Gábor Mezo, Péter Kele, Otto S. Wolfbeis

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

(Chemical Equation Presented) Selective double click labeling: The determination of nanomolar concentrations of matrix metalloproteinase II is made possible through the use of a nanoprobe that consists of fluorescently doped silica nanoparticles to which a FRET-based enzyme substrate has been conjugated through sequential click reactions.

Original languageEnglish
Pages (from-to)2316-2320
Number of pages5
JournalChemBioChem
Volume10
Issue number14
DOIs
Publication statusPublished - Sep 21 2009

Keywords

  • Click chemistry
  • FRET
  • Fluorescence
  • MMP-2
  • Nanoparticles
  • Silica

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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