Pro-oxidative versus antioxidative reactions between Trolox and Cr(VI): The role of H2O2

Borut Poljšak, Zoltán Gazdag, M. Pesti, Špela Jenko-Brinovec, J. Belágyi, Stojan Plesničar, Peter Raspor

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effect of the Vitamin E model compound Trolox in reactions with Cr(VI) in the presence or absence of hydrogen peroxide was investigated. The aim of this study was to establish and discuss potential Trolox-mediated pro-oxidative reactions. The importance of the Trolox:Cr(VI) ratio in the Cr(VI) reduction process was determined from the EPR spectra and DNA cleavage reactions. In the absence of hydrogen peroxide, reduction of Cr(VI) occurred with concomitant oxidation of Trolox to the phenoxyl radical. In the presence of hydrogen peroxide, Cr(V), produced by the reduction of Cr(VI), reduced hydrogen peroxide to the hydroxyl radical. The latter was detected by spin-trapping the methyl radical following reaction with N-methyl sulfoxide. During Cr(VI) reduction with Trolox, DNA single- or double-strand breaks due to Trolox radical formation were not observed. Relaxed DNA appeared only when H2O2 was added to Trolox/Cr(VI) mixtures most probably due to hydroxyl radical formation during the redox cycling of Cr(V/IV)-species. Fenton-like reactions do not play a significant role in the Trolox/Cr(VI) system in the absence of added H2O2.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalEnvironmental Toxicology and Pharmacology
Volume22
Issue number1
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Hydrogen Peroxide
sulfoxide
Hydroxyl Radical
DNA
Spin Trapping
DNA Cleavage
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
chromium hexavalent ion
Vitamin E
Oxidation-Reduction
Paramagnetic resonance
Oxidation

Keywords

  • Chromium(VI)
  • DNA damage
  • Free radicals
  • Oxidative stress
  • Reactive oxygen species
  • Trolox

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Pro-oxidative versus antioxidative reactions between Trolox and Cr(VI) : The role of H2O2. / Poljšak, Borut; Gazdag, Zoltán; Pesti, M.; Jenko-Brinovec, Špela; Belágyi, J.; Plesničar, Stojan; Raspor, Peter.

In: Environmental Toxicology and Pharmacology, Vol. 22, No. 1, 07.2006, p. 15-19.

Research output: Contribution to journalArticle

Poljšak, Borut ; Gazdag, Zoltán ; Pesti, M. ; Jenko-Brinovec, Špela ; Belágyi, J. ; Plesničar, Stojan ; Raspor, Peter. / Pro-oxidative versus antioxidative reactions between Trolox and Cr(VI) : The role of H2O2. In: Environmental Toxicology and Pharmacology. 2006 ; Vol. 22, No. 1. pp. 15-19.
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