Primary structure of human matrilin-2, chromosome location of the MATN2 gene and conservation of an AT-AC intron in matrilin genes

S. Muratoglu, K. Krysan, M. Balázs, H. Sheng, R. Zákány, L. Módis, I. Kiss, Ferenc Deák

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We isolated full-length cDNA clones for human matrilin-2, an oligomeric protein, which forms filamentous networks in the extracellular matrices of various tissues. The human matrilin-2 precursor is encoded by a 4.0-kb mRNA, it consists of 956 amino acids and shows 93% similarity to the mouse protein. Out of the two von Willebrand factor type A-like domains, the 10 epidermal growth factor-type modules, one unique sequence and the oligomerisation module, the first A domain is the most conserved. RT-PCR demonstrated wide expression of the gene in human cell lines of fibroblastic or epithelial origin. Alternative splicing affected only 19 amino acids in a 75-moiety-long segment, unique to matrilin-2. Isolation and analysis of the 3′ end of the gene revealed that the reason for alternative splicing is alternative 3′ splice site selection. Further, we identified in the human matrilin-2 gene a U12 type AT-AC intron between the last two exons encoding the oligomerisation domain. We mapped the matrilin-2 gene (MATN2) by fluorescence in situ hybridization at chromosome position 8q22.

Original languageEnglish
Pages (from-to)323-327
Number of pages5
JournalCytogenetics and Cell Genetics
Volume90
Issue number3-4
Publication statusPublished - 2000

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Matrilin Proteins
Introns
Chromosomes
RNA Splice Sites
Alternative Splicing
Genes
Amino Acids
von Willebrand Factor
Fluorescence In Situ Hybridization
Epidermal Growth Factor
Extracellular Matrix
Exons
Complementary DNA
Clone Cells
Gene Expression
Cell Line
Polymerase Chain Reaction
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Primary structure of human matrilin-2, chromosome location of the MATN2 gene and conservation of an AT-AC intron in matrilin genes. / Muratoglu, S.; Krysan, K.; Balázs, M.; Sheng, H.; Zákány, R.; Módis, L.; Kiss, I.; Deák, Ferenc.

In: Cytogenetics and Cell Genetics, Vol. 90, No. 3-4, 2000, p. 323-327.

Research output: Contribution to journalArticle

Muratoglu, S. ; Krysan, K. ; Balázs, M. ; Sheng, H. ; Zákány, R. ; Módis, L. ; Kiss, I. ; Deák, Ferenc. / Primary structure of human matrilin-2, chromosome location of the MATN2 gene and conservation of an AT-AC intron in matrilin genes. In: Cytogenetics and Cell Genetics. 2000 ; Vol. 90, No. 3-4. pp. 323-327.
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AU - Balázs, M.

AU - Sheng, H.

AU - Zákány, R.

AU - Módis, L.

AU - Kiss, I.

AU - Deák, Ferenc

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