Primary sources and immunological prerequisites for sST2 secretion in humans

Michael Mildner, Angela Storka, Michael Lichtenauer, Veronika Mlitz, Minoo Ghannadan, Konrad Hoetzenecker, Stefanie Nickl, B. Döme, Erwin Tschachler, Hendrik Jan Ankersmit

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Aims Serum levels of the soluble growth stimulation gene-2 (sST2) are elevated in heart and pulmonary diseases. However, the relationship of the sST2/interleukin (IL)-33 axis and its triggers as well as its organ distribution is still not known. This study was thus designed to investigate the cellular origin and regulation of sST2 and IL-33 in vitro and in vivo. Methods and results sST2 and IL-33 gene expression and protein secretion were analysed in pooled organ-specific cDNAs and in primary cell cultures, respectively, by RT-PCR and ELISA technology. The strongest sST2 mRNA expression was detected in heart and lung tissues, which correlated with spontaneous secretion of sST2 protein in vitro. The inflammatory cytokines IL-1, IL-1, and tumour necrosis factor as well as supernatants of lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells led to an enhanced secretion of sST2 in cultured cardiac myocytes and lung alveolar epithelial cells. These cytokines enhanced sST2 secretion via an NFκB-dependent mechanism. In addition, LPS stimulation in humans in vivo induced a short-term inflammatory response that was followed by a massive enhancement of sST2 secretion. Conclusion These results identify the primary sources and inflammatory triggers for the enhancement of sST2 secretion and demonstrate a relationship between inflammation and the secretion of a bioactive member of the IL-1R family, both in vitro and in vivo.

Original languageEnglish
Pages (from-to)769-777
Number of pages9
JournalCardiovascular Research
Volume87
Issue number4
DOIs
Publication statusPublished - Sep 1 2010

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Interleukin-1
Lipopolysaccharides
Cytokines
Alveolar Epithelial Cells
Pulmonary Heart Disease
Lung
Primary Cell Culture
Interleukins
Cardiac Myocytes
Blood Cells
Proteins
Complementary DNA
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Inflammation
Technology
Gene Expression
Polymerase Chain Reaction
Messenger RNA
Growth

Keywords

  • Alveolar epithelial cells
  • Cardiac myocytes
  • Cell culture
  • Inflammation
  • Soluble growth stimulation gene-2

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Physiology

Cite this

Mildner, M., Storka, A., Lichtenauer, M., Mlitz, V., Ghannadan, M., Hoetzenecker, K., ... Ankersmit, H. J. (2010). Primary sources and immunological prerequisites for sST2 secretion in humans. Cardiovascular Research, 87(4), 769-777. https://doi.org/10.1093/cvr/cvq104

Primary sources and immunological prerequisites for sST2 secretion in humans. / Mildner, Michael; Storka, Angela; Lichtenauer, Michael; Mlitz, Veronika; Ghannadan, Minoo; Hoetzenecker, Konrad; Nickl, Stefanie; Döme, B.; Tschachler, Erwin; Ankersmit, Hendrik Jan.

In: Cardiovascular Research, Vol. 87, No. 4, 01.09.2010, p. 769-777.

Research output: Contribution to journalArticle

Mildner, M, Storka, A, Lichtenauer, M, Mlitz, V, Ghannadan, M, Hoetzenecker, K, Nickl, S, Döme, B, Tschachler, E & Ankersmit, HJ 2010, 'Primary sources and immunological prerequisites for sST2 secretion in humans', Cardiovascular Research, vol. 87, no. 4, pp. 769-777. https://doi.org/10.1093/cvr/cvq104
Mildner M, Storka A, Lichtenauer M, Mlitz V, Ghannadan M, Hoetzenecker K et al. Primary sources and immunological prerequisites for sST2 secretion in humans. Cardiovascular Research. 2010 Sep 1;87(4):769-777. https://doi.org/10.1093/cvr/cvq104
Mildner, Michael ; Storka, Angela ; Lichtenauer, Michael ; Mlitz, Veronika ; Ghannadan, Minoo ; Hoetzenecker, Konrad ; Nickl, Stefanie ; Döme, B. ; Tschachler, Erwin ; Ankersmit, Hendrik Jan. / Primary sources and immunological prerequisites for sST2 secretion in humans. In: Cardiovascular Research. 2010 ; Vol. 87, No. 4. pp. 769-777.
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