Prevention of indomethacin-induced acute gastric mucosal injury by spermine in the rat

Gy Buzás, Zs Demel, J. Józan, J. Hamar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of spermine tetrahydrochloride was investigated on the indomethacin-induced acute mucosal injury and mucosal lipid peroxidation in rats. Spermine was given orally at doses of 25, 75 and 150 mg/kg and, intraperitoneally at 10, 25 and 50 mg/kg 1 hour before 25 mg/kg indomethacin administration. Macroscopic injury was measured by the use of a stereomicroscope. Mucosal malondialdehyde (MDA) levels were determined by an HPLC method. Oral spermine reduced dose-dependently the extent of macroscopic injury from 22.87±2.88 mm to 11.8±2.48 mm (p<0 01, 25 mg/kg), 6.53±4.19 mm (p<0.0007, 75 mg/kg) and 0.25±0.25 mm (p<0.0001,150 mg/kg). Intraperitoneally administered spermine prevented dose-dependently the indomethacin produced lesions from 19.25±4.31 mm to 12.63±3.18 mm (p<0.01, 25 mg/kg) and to 0.33±0.33 mm (p<0.001, 50 mg/kg). Doses necessary for intraperitoneal protection were lower than those achieving similar effect, orally suggesting different mechanisms of action. Neither oral or intraperitoneal spermine influenced the mucosal MDA levels.

Original languageEnglish
Pages (from-to)251-258
Number of pages8
JournalActa physiologica Hungarica
Volume84
Issue number3
Publication statusPublished - Dec 1 1996

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Keywords

  • Acute gastric mucosal injury
  • Free radicals
  • Indomethacin
  • Malondialdehyde
  • Polyamine
  • Spermine

ASJC Scopus subject areas

  • Physiology (medical)

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