The effect of spermine tetrahydrochloride was investigated on the indomethacin-induced acute mucosal injury and mucosal lipid peroxidation in rats. Spermine was given orally at doses of 25, 75 and 150 mg/kg and, intraperitoneally at 10, 25 and 50 mg/kg 1 hour before 25 mg/kg indomethacin administration. Macroscopic injury was measured by the use of a stereomicroscope. Mucosal malondialdehyde (MDA) levels were determined by an HPLC method. Oral spermine reduced dose-dependently the extent of macroscopic injury from 22.87±2.88 mm to 11.8±2.48 mm (p<0 01, 25 mg/kg), 6.53±4.19 mm (p<0.0007, 75 mg/kg) and 0.25±0.25 mm (p<0.0001,150 mg/kg). Intraperitoneally administered spermine prevented dose-dependently the indomethacin produced lesions from 19.25±4.31 mm to 12.63±3.18 mm (p<0.01, 25 mg/kg) and to 0.33±0.33 mm (p<0.001, 50 mg/kg). Doses necessary for intraperitoneal protection were lower than those achieving similar effect, orally suggesting different mechanisms of action. Neither oral or intraperitoneal spermine influenced the mucosal MDA levels.
|Number of pages||8|
|Journal||Acta physiologica Hungarica|
|Publication status||Published - Dec 1 1996|
- Acute gastric mucosal injury
- Free radicals
ASJC Scopus subject areas
- Physiology (medical)