INTRODUCTION - Apoptosis has been shown in the literature to be a special form of cell death a gene-regulated cell-suicide basically different from necrosis. It occurs in renal tubular epithelial cells during the reperfusion phase after brief periods of renal ischaemia which is very important in the development of renal failure after transplantation of a cadaver kidney. MATERIAL AND METHODS - In the present study apoptosis was examined in the rat kidney in the first 48 hours after a 30 minute ischaemia. In the first series apoptotic indices were calculated 0, 1, 2, 4, 6, 24 and 48 hours after reperfusion. In the second series apoptosis was influenced by the administration of Ca-channel blockers (verapamil, bepridil, nifedipine and fendilin), and indices were calculated at 6 hours after reperfusion. RESULTS - Apoptosis was first observed two hours after the start of reperfusion in the renal tubular cells and reached a maximum at the 6th hour. On various sites of the tubular epithel significantly different apoptotic indices were calculated. All the administrated Ca-channel blockers decreased the occurrence of apoptosis. CONCLUSION - These results proved that Ca- channel blockers have an advantageous effect in the prevention of the reperfusion injury through the reduction of apoptosis.
|Translated title of the contribution||Prevention of apoptosis reperfusion renal injury by calcium channel blockers|
|Number of pages||5|
|Journal||Lege Artis Medicinae|
|Publication status||Published - Jul 8 1998|
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