The effect of cycloheximide on neutral red-induced autophagocytosis in pancreatic acinar cells of the mouse in vivo was investigated. Administration of the dye resulted in an appearance of basophilic granules (Chlopin's krinom granules) in the cytoplasm. The cells containing krinom granules were always found to contain a great number of autophagic vacuoles in various stages of degradation. It was concluded, that the krinom granules are light microscopic indicators of autophagy in this cell type. Cycloheximide in doses of 0.1 mg/g body weight and 0.05 mg/g b.w. given simultaneously with or prior to neutral red was found to prevent the dye-induced formation of autophagic vacuoles and krinom granules as well. Evidence was obtained that it was not due to an inhibition of the cellular uptake of neutral red. The protective effect of the drug might be related to its capability for preserving the ultrastructural integrity of the endoplasmic reticulum.
ASJC Scopus subject areas
- Pathology and Forensic Medicine