Prevalence of human papillomaviruses in the healthy oral mucosa of women with high-grade squamous intra-epithelial lesion and of their partners as compared to healthy controls

Tímea Zsófia Tatár, Andrea Kis, Éva Szabó, Levente Czompa, Róbert Boda, Ildikó Tar, K. Szarka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Oral human papillomavirus (HPV) carriage rates were investigated in relation to genital HPV carriage in women with HPV-associated cervical lesions and male partner of such women, including several couples, in comparison with healthy individuals. Buccal and lingual mucosa of 60 males and 149 females with healthy oral mucosa and without known genital lesion, genital and oral mucosa of further 40 females with cervical high-grade squamous intraepithelial lesion (HSIL) and 34 male sexual partners of women with HSIL (including 20 couples) were sampled. HPV DNA was detected using MY/GP PCR. Genotype was determined by sequencing or restriction fragment length polymorphism. Virus copy numbers were determined by real-time PCR. Overall, oral HPV carriage rate was 5.7% (12/209) in healthy individuals; average copy number was 5.8 × 102 copies/1 μg DNA; male and female rates were comparable. Oral carriage in women with HSIL was significantly higher, 20.0% (8/40, P = 0.003); males with partners with HSIL showed a carriage rate of 17.6% (6/34), copy numbers were similar to the healthy controls. In contrast, genital carriage rate (52.9%, 18/34 vs. 82.5%, 33/40; P = 0.006) and average copy number were lower in males (5.0 × 105 vs. 7.8 × 105 copies/1 μg DNA; P = 0.01). Oral copy numbers in these groups and in healthy individuals were comparable. High-risk genotypes were dominant; couples usually had the same genotype in the genital sample. In conclusion, genital HPV carriage is a risk factor of oral carriage for the individual or for the sexual partner, but alone is not sufficient to produce an oral HPV infection in most cases.

Original languageEnglish
Pages (from-to)722-727
Number of pages6
JournalJournal of Oral Pathology and Medicine
Volume44
Issue number9
DOIs
Publication statusPublished - Oct 1 2015

Fingerprint

Mouth Mucosa
Sexual Partners
Genotype
DNA
Papillomavirus Infections
Tongue
Restriction Fragment Length Polymorphisms
Real-Time Polymerase Chain Reaction
Viruses
Polymerase Chain Reaction
Squamous Intraepithelial Lesions of the Cervix

Keywords

  • Asymptomatic human papillomavirus carriage
  • Genital human papillomavirus infection
  • Human papillomavirus transmission
  • Virus copy numbers

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics

Cite this

Prevalence of human papillomaviruses in the healthy oral mucosa of women with high-grade squamous intra-epithelial lesion and of their partners as compared to healthy controls. / Tatár, Tímea Zsófia; Kis, Andrea; Szabó, Éva; Czompa, Levente; Boda, Róbert; Tar, Ildikó; Szarka, K.

In: Journal of Oral Pathology and Medicine, Vol. 44, No. 9, 01.10.2015, p. 722-727.

Research output: Contribution to journalArticle

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abstract = "Oral human papillomavirus (HPV) carriage rates were investigated in relation to genital HPV carriage in women with HPV-associated cervical lesions and male partner of such women, including several couples, in comparison with healthy individuals. Buccal and lingual mucosa of 60 males and 149 females with healthy oral mucosa and without known genital lesion, genital and oral mucosa of further 40 females with cervical high-grade squamous intraepithelial lesion (HSIL) and 34 male sexual partners of women with HSIL (including 20 couples) were sampled. HPV DNA was detected using MY/GP PCR. Genotype was determined by sequencing or restriction fragment length polymorphism. Virus copy numbers were determined by real-time PCR. Overall, oral HPV carriage rate was 5.7{\%} (12/209) in healthy individuals; average copy number was 5.8 × 102 copies/1 μg DNA; male and female rates were comparable. Oral carriage in women with HSIL was significantly higher, 20.0{\%} (8/40, P = 0.003); males with partners with HSIL showed a carriage rate of 17.6{\%} (6/34), copy numbers were similar to the healthy controls. In contrast, genital carriage rate (52.9{\%}, 18/34 vs. 82.5{\%}, 33/40; P = 0.006) and average copy number were lower in males (5.0 × 105 vs. 7.8 × 105 copies/1 μg DNA; P = 0.01). Oral copy numbers in these groups and in healthy individuals were comparable. High-risk genotypes were dominant; couples usually had the same genotype in the genital sample. In conclusion, genital HPV carriage is a risk factor of oral carriage for the individual or for the sexual partner, but alone is not sufficient to produce an oral HPV infection in most cases.",
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