Prevalence of functional haplotypes of the peptidylarginine deiminase citrullinating enzyme gene in patients with rheumatoid arthritis: No influence of the presence of anti-citrullinated peptide antibodies

Bernadett Faragó, G. Talián, A. Maász, L. Magyari, Katalin Horvatovich, Beáta Kovács, Veronika Cserép, P. Kisfali, C. Kiss, László Czirjḱ, B. Melegh

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Abstract

Objective: Citrullinated peptides produced by enzymatic deimination of arginine residues in proteins by peptidylarginine deiminases are of particular interest in the pathogenesis of rheumatoid arthritis (RA). One type of citrullinated protein - the cyclic citrullinated peptide - is the target of the anti-cyclic citrullinated peptide antibody, the most sensitive and specific autoantibody in RA. The peptidylarginine deiminase type 4 (PAD14) gene, which codes one of the PADI enzyme isotypes, has genetic variants that confer susceptibility to RA in Asian, but not in European populations. Methods: Genetic associations were examined in 214 Hungarian RA patients characterized for the presence of anti-CCP and rheumatoid factor. The patients were characterized for the existing haplotypes of the PAD14 gene (defined by the combinations of 4 exonic padi4_89: 163G/A, padi4_90: 245T/C, padi4_92: 335C/G, padi4_104: 349T/C and 2 intronic padi4_94: 17535226C/T and padi4_102: 17546809C/T variants) by the PCR-RFLP method. Results: None of the PAD14 haplotypes was accumulated in RA patients. One new finding was that we also did not detect the accumulation of any haplotypes either in the anti-CCP or in the RF-positive subgroups of patients. Conclusion: The data presented here show that none of the naturally occurring haplotypes of the PAD14 gene conferred susceptibility to RA in an average group of Hungarian patients; this is in agreement with findings for other European populations. In addition, none of the functional PAD14 haplotypes were associated with the pathologic immune response, which was evidenced by the absence of accumulation of anti-CCP-positive subjects in the specific PAD14 haplotypes.

Original languageEnglish
Pages (from-to)523-528
Number of pages6
JournalClinical and Experimental Rheumatology
Volume25
Issue number4
Publication statusPublished - Jul 2007

Fingerprint

Haplotypes
Rheumatoid Arthritis
Peptides
Antibodies
Enzymes
Genes
Rheumatoid Factor
Restriction Fragment Length Polymorphisms
Autoantibodies
Population
Arginine
protein-arginine deiminase
Proteins
Polymerase Chain Reaction
cyclic citrullinated peptide

Keywords

  • Anti-CCP
  • Caucasian
  • Haplotype
  • PADI4
  • RF
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

@article{e300989a26854e739d8d8a57a161d7d9,
title = "Prevalence of functional haplotypes of the peptidylarginine deiminase citrullinating enzyme gene in patients with rheumatoid arthritis: No influence of the presence of anti-citrullinated peptide antibodies",
abstract = "Objective: Citrullinated peptides produced by enzymatic deimination of arginine residues in proteins by peptidylarginine deiminases are of particular interest in the pathogenesis of rheumatoid arthritis (RA). One type of citrullinated protein - the cyclic citrullinated peptide - is the target of the anti-cyclic citrullinated peptide antibody, the most sensitive and specific autoantibody in RA. The peptidylarginine deiminase type 4 (PAD14) gene, which codes one of the PADI enzyme isotypes, has genetic variants that confer susceptibility to RA in Asian, but not in European populations. Methods: Genetic associations were examined in 214 Hungarian RA patients characterized for the presence of anti-CCP and rheumatoid factor. The patients were characterized for the existing haplotypes of the PAD14 gene (defined by the combinations of 4 exonic padi4_89: 163G/A, padi4_90: 245T/C, padi4_92: 335C/G, padi4_104: 349T/C and 2 intronic padi4_94: 17535226C/T and padi4_102: 17546809C/T variants) by the PCR-RFLP method. Results: None of the PAD14 haplotypes was accumulated in RA patients. One new finding was that we also did not detect the accumulation of any haplotypes either in the anti-CCP or in the RF-positive subgroups of patients. Conclusion: The data presented here show that none of the naturally occurring haplotypes of the PAD14 gene conferred susceptibility to RA in an average group of Hungarian patients; this is in agreement with findings for other European populations. In addition, none of the functional PAD14 haplotypes were associated with the pathologic immune response, which was evidenced by the absence of accumulation of anti-CCP-positive subjects in the specific PAD14 haplotypes.",
keywords = "Anti-CCP, Caucasian, Haplotype, PADI4, RF, Rheumatoid arthritis",
author = "Bernadett Farag{\'o} and G. Tali{\'a}n and A. Ma{\'a}sz and L. Magyari and Katalin Horvatovich and Be{\'a}ta Kov{\'a}cs and Veronika Cser{\'e}p and P. Kisfali and C. Kiss and L{\'a}szl{\'o} Czirjḱ and B. Melegh",
year = "2007",
month = "7",
language = "English",
volume = "25",
pages = "523--528",
journal = "Clinical and Experimental Rheumatology",
issn = "0392-856X",
publisher = "Clinical and Experimental Rheumatology S.A.S.",
number = "4",

}

TY - JOUR

T1 - Prevalence of functional haplotypes of the peptidylarginine deiminase citrullinating enzyme gene in patients with rheumatoid arthritis

T2 - No influence of the presence of anti-citrullinated peptide antibodies

AU - Faragó, Bernadett

AU - Talián, G.

AU - Maász, A.

AU - Magyari, L.

AU - Horvatovich, Katalin

AU - Kovács, Beáta

AU - Cserép, Veronika

AU - Kisfali, P.

AU - Kiss, C.

AU - Czirjḱ, László

AU - Melegh, B.

PY - 2007/7

Y1 - 2007/7

N2 - Objective: Citrullinated peptides produced by enzymatic deimination of arginine residues in proteins by peptidylarginine deiminases are of particular interest in the pathogenesis of rheumatoid arthritis (RA). One type of citrullinated protein - the cyclic citrullinated peptide - is the target of the anti-cyclic citrullinated peptide antibody, the most sensitive and specific autoantibody in RA. The peptidylarginine deiminase type 4 (PAD14) gene, which codes one of the PADI enzyme isotypes, has genetic variants that confer susceptibility to RA in Asian, but not in European populations. Methods: Genetic associations were examined in 214 Hungarian RA patients characterized for the presence of anti-CCP and rheumatoid factor. The patients were characterized for the existing haplotypes of the PAD14 gene (defined by the combinations of 4 exonic padi4_89: 163G/A, padi4_90: 245T/C, padi4_92: 335C/G, padi4_104: 349T/C and 2 intronic padi4_94: 17535226C/T and padi4_102: 17546809C/T variants) by the PCR-RFLP method. Results: None of the PAD14 haplotypes was accumulated in RA patients. One new finding was that we also did not detect the accumulation of any haplotypes either in the anti-CCP or in the RF-positive subgroups of patients. Conclusion: The data presented here show that none of the naturally occurring haplotypes of the PAD14 gene conferred susceptibility to RA in an average group of Hungarian patients; this is in agreement with findings for other European populations. In addition, none of the functional PAD14 haplotypes were associated with the pathologic immune response, which was evidenced by the absence of accumulation of anti-CCP-positive subjects in the specific PAD14 haplotypes.

AB - Objective: Citrullinated peptides produced by enzymatic deimination of arginine residues in proteins by peptidylarginine deiminases are of particular interest in the pathogenesis of rheumatoid arthritis (RA). One type of citrullinated protein - the cyclic citrullinated peptide - is the target of the anti-cyclic citrullinated peptide antibody, the most sensitive and specific autoantibody in RA. The peptidylarginine deiminase type 4 (PAD14) gene, which codes one of the PADI enzyme isotypes, has genetic variants that confer susceptibility to RA in Asian, but not in European populations. Methods: Genetic associations were examined in 214 Hungarian RA patients characterized for the presence of anti-CCP and rheumatoid factor. The patients were characterized for the existing haplotypes of the PAD14 gene (defined by the combinations of 4 exonic padi4_89: 163G/A, padi4_90: 245T/C, padi4_92: 335C/G, padi4_104: 349T/C and 2 intronic padi4_94: 17535226C/T and padi4_102: 17546809C/T variants) by the PCR-RFLP method. Results: None of the PAD14 haplotypes was accumulated in RA patients. One new finding was that we also did not detect the accumulation of any haplotypes either in the anti-CCP or in the RF-positive subgroups of patients. Conclusion: The data presented here show that none of the naturally occurring haplotypes of the PAD14 gene conferred susceptibility to RA in an average group of Hungarian patients; this is in agreement with findings for other European populations. In addition, none of the functional PAD14 haplotypes were associated with the pathologic immune response, which was evidenced by the absence of accumulation of anti-CCP-positive subjects in the specific PAD14 haplotypes.

KW - Anti-CCP

KW - Caucasian

KW - Haplotype

KW - PADI4

KW - RF

KW - Rheumatoid arthritis

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JO - Clinical and Experimental Rheumatology

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