Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

Ann Nordengrahn, Malik Merza, Carlos Ros, Arne Lindholm, V. Pálfi, Duncan Hannant, Sándor Belák

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) have a rather unclear pathogenicity and distribution within the equid population. In order to gain more information on the prevalence of these two viruses, type-specific PCR assays were developed to detect viral DNA in nasal specimens and in peripheral blood leukocytes (PBLs) of adult horses and foals from various regions of Europe, i.e. Sweden, Hungary and the United Kingdom. In adult horses, the prevalence of EHV-2 in PBLs was up to 68% in Sweden and 71% in the United Kingdom. EHV-2 DNA was detected in the PBLs from all the foals tested in all countries and most (93%) of the nasal specimens also yielded positive results. The prevalence of EHV-5 DNA in the PBLs of foals in Hungary was 15 and 24% in adult horses in the United Kingdom. This observation was among the very few reports of the presence of EHV-5 in horses. In summary, the specific PCR assays revealed important data on the occurrence and distribution of EHV-2 and EHV-5 in large horse populations. The findings indicated that infection with EHV-5 occurred later than EHV-2 in foals. This study may contribute to a better understanding of the etiological role of these gammaherpesviruses in equine diseases.

Original languageEnglish
Pages (from-to)251-259
Number of pages9
JournalVeterinary Research
Volume33
Issue number3
Publication statusPublished - 2002

Fingerprint

Rhadinovirus
Equid herpesvirus 5
Equid herpesvirus 2
Horses
horses
foals
Leukocytes
Polymerase Chain Reaction
leukocytes
assays
Hungary
Population
United Kingdom
Nose
Sweden
blood
Horse Diseases
DNA
Viral DNA
horse diseases

Keywords

  • EHV-2
  • EHV-5
  • Equine infections
  • Gammaherpesvirinae
  • PCR

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Nordengrahn, A., Merza, M., Ros, C., Lindholm, A., Pálfi, V., Hannant, D., & Belák, S. (2002). Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays. Veterinary Research, 33(3), 251-259.

Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays. / Nordengrahn, Ann; Merza, Malik; Ros, Carlos; Lindholm, Arne; Pálfi, V.; Hannant, Duncan; Belák, Sándor.

In: Veterinary Research, Vol. 33, No. 3, 2002, p. 251-259.

Research output: Contribution to journalArticle

Nordengrahn, A, Merza, M, Ros, C, Lindholm, A, Pálfi, V, Hannant, D & Belák, S 2002, 'Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays', Veterinary Research, vol. 33, no. 3, pp. 251-259.
Nordengrahn, Ann ; Merza, Malik ; Ros, Carlos ; Lindholm, Arne ; Pálfi, V. ; Hannant, Duncan ; Belák, Sándor. / Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays. In: Veterinary Research. 2002 ; Vol. 33, No. 3. pp. 251-259.
@article{fb04d363b8ee4e559eda379a0c71979c,
title = "Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays",
abstract = "Equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) have a rather unclear pathogenicity and distribution within the equid population. In order to gain more information on the prevalence of these two viruses, type-specific PCR assays were developed to detect viral DNA in nasal specimens and in peripheral blood leukocytes (PBLs) of adult horses and foals from various regions of Europe, i.e. Sweden, Hungary and the United Kingdom. In adult horses, the prevalence of EHV-2 in PBLs was up to 68{\%} in Sweden and 71{\%} in the United Kingdom. EHV-2 DNA was detected in the PBLs from all the foals tested in all countries and most (93{\%}) of the nasal specimens also yielded positive results. The prevalence of EHV-5 DNA in the PBLs of foals in Hungary was 15 and 24{\%} in adult horses in the United Kingdom. This observation was among the very few reports of the presence of EHV-5 in horses. In summary, the specific PCR assays revealed important data on the occurrence and distribution of EHV-2 and EHV-5 in large horse populations. The findings indicated that infection with EHV-5 occurred later than EHV-2 in foals. This study may contribute to a better understanding of the etiological role of these gammaherpesviruses in equine diseases.",
keywords = "EHV-2, EHV-5, Equine infections, Gammaherpesvirinae, PCR",
author = "Ann Nordengrahn and Malik Merza and Carlos Ros and Arne Lindholm and V. P{\'a}lfi and Duncan Hannant and S{\'a}ndor Bel{\'a}k",
year = "2002",
language = "English",
volume = "33",
pages = "251--259",
journal = "Annales de Recherches Veterinaires",
issn = "0928-4249",
publisher = "BioMed Central",
number = "3",

}

TY - JOUR

T1 - Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays

AU - Nordengrahn, Ann

AU - Merza, Malik

AU - Ros, Carlos

AU - Lindholm, Arne

AU - Pálfi, V.

AU - Hannant, Duncan

AU - Belák, Sándor

PY - 2002

Y1 - 2002

N2 - Equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) have a rather unclear pathogenicity and distribution within the equid population. In order to gain more information on the prevalence of these two viruses, type-specific PCR assays were developed to detect viral DNA in nasal specimens and in peripheral blood leukocytes (PBLs) of adult horses and foals from various regions of Europe, i.e. Sweden, Hungary and the United Kingdom. In adult horses, the prevalence of EHV-2 in PBLs was up to 68% in Sweden and 71% in the United Kingdom. EHV-2 DNA was detected in the PBLs from all the foals tested in all countries and most (93%) of the nasal specimens also yielded positive results. The prevalence of EHV-5 DNA in the PBLs of foals in Hungary was 15 and 24% in adult horses in the United Kingdom. This observation was among the very few reports of the presence of EHV-5 in horses. In summary, the specific PCR assays revealed important data on the occurrence and distribution of EHV-2 and EHV-5 in large horse populations. The findings indicated that infection with EHV-5 occurred later than EHV-2 in foals. This study may contribute to a better understanding of the etiological role of these gammaherpesviruses in equine diseases.

AB - Equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) have a rather unclear pathogenicity and distribution within the equid population. In order to gain more information on the prevalence of these two viruses, type-specific PCR assays were developed to detect viral DNA in nasal specimens and in peripheral blood leukocytes (PBLs) of adult horses and foals from various regions of Europe, i.e. Sweden, Hungary and the United Kingdom. In adult horses, the prevalence of EHV-2 in PBLs was up to 68% in Sweden and 71% in the United Kingdom. EHV-2 DNA was detected in the PBLs from all the foals tested in all countries and most (93%) of the nasal specimens also yielded positive results. The prevalence of EHV-5 DNA in the PBLs of foals in Hungary was 15 and 24% in adult horses in the United Kingdom. This observation was among the very few reports of the presence of EHV-5 in horses. In summary, the specific PCR assays revealed important data on the occurrence and distribution of EHV-2 and EHV-5 in large horse populations. The findings indicated that infection with EHV-5 occurred later than EHV-2 in foals. This study may contribute to a better understanding of the etiological role of these gammaherpesviruses in equine diseases.

KW - EHV-2

KW - EHV-5

KW - Equine infections

KW - Gammaherpesvirinae

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=0036264890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036264890&partnerID=8YFLogxK

M3 - Article

VL - 33

SP - 251

EP - 259

JO - Annales de Recherches Veterinaires

JF - Annales de Recherches Veterinaires

SN - 0928-4249

IS - 3

ER -