Prevalence, nucleotide sequence and expression studies of two proteins of a 5.6kb, Class III, Bacteroides plasmid frequently found in clinical isolates from European countries

J. Sóki, David W. Wareham, Csilla Rátkai, Joseph Aduse-Opoku, E. Urbán, E. Nagy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In order to gain information on the significance and functions of small molecular weight plasmids (usually regarded as cryptic) of Bacteroides isolates, we screened 178 European clinical Bacteroides isolates for plasmids and determined the nucleotide sequence of a 5.6kb plasmid. The previously observed frequent plasmid types (Classes I-III; 2.7, 4.2 and 5.6kb, respectively) were found to exhibit different distributions in five European countries as concerns plasmid type, geographical location, taxonomy and time course. The Class I plasmids displayed markedly different distribution properties from those of the Class II and III plasmids. The nucleotide sequence of a representative of the most frequent Class III plasmids, pBFP35, originating from Hungary, was determined (5594bp) and analyzed. A total of eight open reading frames (ORFs) were annotated, of which four proved to participate unequivocally in such plasmid maintenance functions as replication (repAP35), mobilization (mobAP35) and stability (mazEP35 and mazFP35). Four additional ORFs (orf1-4) were identified. Orf1 was predicted to code a lipoprotein. In expression studies in an Escherichia coli host, Orf1 behaved as a periplasmic protein.

Original languageEnglish
Pages (from-to)86-97
Number of pages12
JournalPlasmid
Volume63
Issue number2
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Bacteroides
Plasmids
Proteins
Open Reading Frames
Periplasmic Proteins
Hungary
Lipoproteins
Molecular Weight
Maintenance
Escherichia coli

Keywords

  • Bacteroides
  • Cryptic plasmid(s)
  • Lipoprotein
  • Plasmid functions

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Prevalence, nucleotide sequence and expression studies of two proteins of a 5.6kb, Class III, Bacteroides plasmid frequently found in clinical isolates from European countries. / Sóki, J.; Wareham, David W.; Rátkai, Csilla; Aduse-Opoku, Joseph; Urbán, E.; Nagy, E.

In: Plasmid, Vol. 63, No. 2, 03.2010, p. 86-97.

Research output: Contribution to journalArticle

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