Prevalence and age distribution of human herpesvirus-8 specific antibodies in Hungarian blood donors

A. Juhász, E. Remenyik, József Kónya, G. Veress, Agnes Bégány, István Andirkó, Ildikó Medgyessy, J. Hunyadi, L. Gergely

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Sera of blood donors were investigated by a peptide ELISA and indirect immunofluorescence assay to assess the prevalence of H HV-8 infection in the Hungarian population. A 14 amino acid long synthetic oligopeptide from the carboxyterminus of orf65/small virus capsid antigen was used as antigen in the ELISA. ELISA results were confirmed by recombinant orf65 antigen Western blot. Antibodies to the latent nuclear antigen were detected by the immunofluorescence assay. Nine of 12 sera obtained from patients with classical Kaposi sarcoma were reactive by ELISA whereas all were positive by immunofluorescence. Four of 482 (0.83%) healthy blood donors had anti-orf65 peptide antibodies and 17/1089 (1.56%) had antibodies to the latent nuclear antigen. In a group of children ages 1-14 years, antibodies to the latent nuclear antigen (0/29) were not detected. The prevalence of antibodies to the latent nuclear antigen showed a moderate but significant increase in correlation with senescence. In the Kaposi sarcoma patients, the titre of antibodies to the latent nuclear antigen was significantly higher than in the healthy seropositive donors. The overall HHV-8 seroprevalence by the two assays was 2.28% (11/482) in the Hungarian blood donor group.

Original languageEnglish
Pages (from-to)526-530
Number of pages5
JournalJournal of Medical Virology
Volume64
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Human Herpesvirus 8
Age Distribution
Blood Donors
Antibodies
Enzyme-Linked Immunosorbent Assay
Kaposi's Sarcoma
Antigens
Fluorescent Antibody Technique
Oligopeptides
Peptides
Capsid
Seroepidemiologic Studies
Indirect Fluorescent Antibody Technique
Blood Group Antigens
Serum
Age Groups
Western Blotting
latent nuclear antigen (LNA)
Tissue Donors
Viruses

Keywords

  • Epidemiology
  • HHV-8
  • Orf65
  • Orf73
  • Peptide ELI-SA
  • Serology

ASJC Scopus subject areas

  • Virology

Cite this

Prevalence and age distribution of human herpesvirus-8 specific antibodies in Hungarian blood donors. / Juhász, A.; Remenyik, E.; Kónya, József; Veress, G.; Bégány, Agnes; Andirkó, István; Medgyessy, Ildikó; Hunyadi, J.; Gergely, L.

In: Journal of Medical Virology, Vol. 64, No. 4, 2001, p. 526-530.

Research output: Contribution to journalArticle

@article{4057640387b3493f9b3962880d52d275,
title = "Prevalence and age distribution of human herpesvirus-8 specific antibodies in Hungarian blood donors",
abstract = "Sera of blood donors were investigated by a peptide ELISA and indirect immunofluorescence assay to assess the prevalence of H HV-8 infection in the Hungarian population. A 14 amino acid long synthetic oligopeptide from the carboxyterminus of orf65/small virus capsid antigen was used as antigen in the ELISA. ELISA results were confirmed by recombinant orf65 antigen Western blot. Antibodies to the latent nuclear antigen were detected by the immunofluorescence assay. Nine of 12 sera obtained from patients with classical Kaposi sarcoma were reactive by ELISA whereas all were positive by immunofluorescence. Four of 482 (0.83{\%}) healthy blood donors had anti-orf65 peptide antibodies and 17/1089 (1.56{\%}) had antibodies to the latent nuclear antigen. In a group of children ages 1-14 years, antibodies to the latent nuclear antigen (0/29) were not detected. The prevalence of antibodies to the latent nuclear antigen showed a moderate but significant increase in correlation with senescence. In the Kaposi sarcoma patients, the titre of antibodies to the latent nuclear antigen was significantly higher than in the healthy seropositive donors. The overall HHV-8 seroprevalence by the two assays was 2.28{\%} (11/482) in the Hungarian blood donor group.",
keywords = "Epidemiology, HHV-8, Orf65, Orf73, Peptide ELI-SA, Serology",
author = "A. Juh{\'a}sz and E. Remenyik and J{\'o}zsef K{\'o}nya and G. Veress and Agnes B{\'e}g{\'a}ny and Istv{\'a}n Andirk{\'o} and Ildik{\'o} Medgyessy and J. Hunyadi and L. Gergely",
year = "2001",
doi = "10.1002/jmv.1081",
language = "English",
volume = "64",
pages = "526--530",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Prevalence and age distribution of human herpesvirus-8 specific antibodies in Hungarian blood donors

AU - Juhász, A.

AU - Remenyik, E.

AU - Kónya, József

AU - Veress, G.

AU - Bégány, Agnes

AU - Andirkó, István

AU - Medgyessy, Ildikó

AU - Hunyadi, J.

AU - Gergely, L.

PY - 2001

Y1 - 2001

N2 - Sera of blood donors were investigated by a peptide ELISA and indirect immunofluorescence assay to assess the prevalence of H HV-8 infection in the Hungarian population. A 14 amino acid long synthetic oligopeptide from the carboxyterminus of orf65/small virus capsid antigen was used as antigen in the ELISA. ELISA results were confirmed by recombinant orf65 antigen Western blot. Antibodies to the latent nuclear antigen were detected by the immunofluorescence assay. Nine of 12 sera obtained from patients with classical Kaposi sarcoma were reactive by ELISA whereas all were positive by immunofluorescence. Four of 482 (0.83%) healthy blood donors had anti-orf65 peptide antibodies and 17/1089 (1.56%) had antibodies to the latent nuclear antigen. In a group of children ages 1-14 years, antibodies to the latent nuclear antigen (0/29) were not detected. The prevalence of antibodies to the latent nuclear antigen showed a moderate but significant increase in correlation with senescence. In the Kaposi sarcoma patients, the titre of antibodies to the latent nuclear antigen was significantly higher than in the healthy seropositive donors. The overall HHV-8 seroprevalence by the two assays was 2.28% (11/482) in the Hungarian blood donor group.

AB - Sera of blood donors were investigated by a peptide ELISA and indirect immunofluorescence assay to assess the prevalence of H HV-8 infection in the Hungarian population. A 14 amino acid long synthetic oligopeptide from the carboxyterminus of orf65/small virus capsid antigen was used as antigen in the ELISA. ELISA results were confirmed by recombinant orf65 antigen Western blot. Antibodies to the latent nuclear antigen were detected by the immunofluorescence assay. Nine of 12 sera obtained from patients with classical Kaposi sarcoma were reactive by ELISA whereas all were positive by immunofluorescence. Four of 482 (0.83%) healthy blood donors had anti-orf65 peptide antibodies and 17/1089 (1.56%) had antibodies to the latent nuclear antigen. In a group of children ages 1-14 years, antibodies to the latent nuclear antigen (0/29) were not detected. The prevalence of antibodies to the latent nuclear antigen showed a moderate but significant increase in correlation with senescence. In the Kaposi sarcoma patients, the titre of antibodies to the latent nuclear antigen was significantly higher than in the healthy seropositive donors. The overall HHV-8 seroprevalence by the two assays was 2.28% (11/482) in the Hungarian blood donor group.

KW - Epidemiology

KW - HHV-8

KW - Orf65

KW - Orf73

KW - Peptide ELI-SA

KW - Serology

UR - http://www.scopus.com/inward/record.url?scp=0034964364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034964364&partnerID=8YFLogxK

U2 - 10.1002/jmv.1081

DO - 10.1002/jmv.1081

M3 - Article

C2 - 11468739

AN - SCOPUS:0034964364

VL - 64

SP - 526

EP - 530

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 4

ER -