Pretreatment with GABA and modulatory ligands enhances GABA receptor binding

G. Maksay, Maharaj K. Ticku

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

GABA pretreatment at 37 ° C but not at 4 ° C enhanced subsequent [3H]GABA binding in a concentration-dependent manner (EC50 = 440 nM) by increasing the number of high and low affinity sites. The effect mediated by GABAA receptors was attenuated by bicuculline methiodide and picrotoxinin and potentiated by pentobarbial, flurazepam and Triton X-100. These results can be interpreted by the facilitated removal of endogenous substances and/or by a temperature-restricted conversion of the GABA receptor populations.

Original languageEnglish
Pages (from-to)185-188
Number of pages4
JournalEuropean Journal of Pharmacology
Volume104
Issue number1-2
DOIs
Publication statusPublished - Sep 3 1984

Fingerprint

GABA Receptors
gamma-Aminobutyric Acid
Flurazepam
Ligands
Octoxynol
GABA-A Receptors
Temperature
Population
bicuculline methiodide
picrotoxinin

Keywords

  • Endogenous substances
  • Enhancement of binding
  • GABA receptor complex
  • Ligand pretreatment
  • Receptor convertibility

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Pretreatment with GABA and modulatory ligands enhances GABA receptor binding. / Maksay, G.; Ticku, Maharaj K.

In: European Journal of Pharmacology, Vol. 104, No. 1-2, 03.09.1984, p. 185-188.

Research output: Contribution to journalArticle

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