Presynaptic release of ATP from superior cervical ganglion of rats modulated by various receptors

Shang Dong Liang, E. Vízi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

AIM: To determine whether adenosine 5'-triphosphate (ATP) is released from the superior cervical ganglion (SCG) of rats and whether the release is regulated by presynaptic mechanism. METHODS: Using the luciferin-luciferase technique. RESULTS: Electric stimulation evoked the release of ATP from the rat SCG. Adenosine (100 μmol·L-1), P1 (A1) purinoceptor agonist N6- cyclopentyladenosine (0.1 μmol·L-1), the muscarinic agonist oxotremorine (1 μmol·L-1), and 5-hydroxytryptamine (100 μmolΛ-1) decreased the evoked release of ATP from the rat SCG. On the contrary, P1 (A1) purinoceptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (10 nmol·L-1), P2 purinoceptor antagonist pyridoxal-5-phosphate-6-azophenyl-2',4'- disulphonic acid (10 μmol·L-1), muscarinic antagonist atropine (1 μmol·L-1), α2 adrenoceptor antagonist yohimbine (3 μmol·L-1), D2 dopamine receptor antagonist sulpiride (20 μmol·L-1), and histamine (100 μmol·L-1) increased the evoked release of ATP from the rat SCG. CONCLUSION: ATP is released from the rat SCG and the release of ATP can be presynaptically modulated by P1 (A1), P2, muscarinic, α2 adrenergic, D2, 5-HT, and H1 receptor agonists and antagonists.

Original languageEnglish
Pages (from-to)589-591
Number of pages3
JournalActa Pharmacologica Sinica
Volume20
Issue number7
Publication statusPublished - 1999

Fingerprint

Superior Cervical Ganglion
Adenosine
Rats
Adenosine Triphosphate
Muscarinic Antagonists
Atropine
Purinergic P2 Receptor Antagonists
Purinergic P1 Receptor Agonists
Histamine Agonists
Purinergic P1 Receptor Antagonists
Purinergic Antagonists
Oxotremorine
Purinergic Agonists
Muscarinic Agonists
Histamine H1 Receptors
Sulpiride
Pyridoxal Phosphate
Yohimbine
Serotonin Receptors
Luciferases

Keywords

  • Adenosine triphosphate
  • Atropine
  • Histamine
  • Oxotremorine
  • Serotonin
  • Sulpiride
  • Superior cervical ganglion
  • Yohimbine

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmacology

Cite this

Presynaptic release of ATP from superior cervical ganglion of rats modulated by various receptors. / Liang, Shang Dong; Vízi, E.

In: Acta Pharmacologica Sinica, Vol. 20, No. 7, 1999, p. 589-591.

Research output: Contribution to journalArticle

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abstract = "AIM: To determine whether adenosine 5'-triphosphate (ATP) is released from the superior cervical ganglion (SCG) of rats and whether the release is regulated by presynaptic mechanism. METHODS: Using the luciferin-luciferase technique. RESULTS: Electric stimulation evoked the release of ATP from the rat SCG. Adenosine (100 μmol·L-1), P1 (A1) purinoceptor agonist N6- cyclopentyladenosine (0.1 μmol·L-1), the muscarinic agonist oxotremorine (1 μmol·L-1), and 5-hydroxytryptamine (100 μmolΛ-1) decreased the evoked release of ATP from the rat SCG. On the contrary, P1 (A1) purinoceptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (10 nmol·L-1), P2 purinoceptor antagonist pyridoxal-5-phosphate-6-azophenyl-2',4'- disulphonic acid (10 μmol·L-1), muscarinic antagonist atropine (1 μmol·L-1), α2 adrenoceptor antagonist yohimbine (3 μmol·L-1), D2 dopamine receptor antagonist sulpiride (20 μmol·L-1), and histamine (100 μmol·L-1) increased the evoked release of ATP from the rat SCG. CONCLUSION: ATP is released from the rat SCG and the release of ATP can be presynaptically modulated by P1 (A1), P2, muscarinic, α2 adrenergic, D2, 5-HT, and H1 receptor agonists and antagonists.",
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