Presynaptic effect of muscle relaxants on the release of3H-norepinephrine controlled by endogenous acetylcholine in guinea pig atrium

F. F. Földes, O. Kobayashi, M. Kinjo, L. Hársing, H. Nagashima, D. Duncalf, P. L. Goldiner, E. Vízi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The influence of nondepolarizing muscle relaxants (MR) on the resting and electrically evoked release of tritiated norepinephrine (3H-NE) was investigated, in the absence and presence of 10-4 mol/l cocaine, in the in vitro right atrium preparation of guinea pigs (g.p.) preloaded with3H-NE. In the absence of MR both resting and stimulated3H and3H-NE release remained relatively constant throughout the experiment and the ratios of the evoked release of3H during consecutive stimulation periods (i.e. S2/S1, S3/S2) were close to unity. None of the MR had any effect on resting3H release. Atropine (3× 10-7 mol/l), gallamine (7×10-5 mol/l), and pancuronium (2×10-6 mol/l), but not d-tubocurarine (5×10-6 mol/l) significantly increased stimulated release of3H-NE. The effect of MR on resting or evoked release of3H-NE was not influenced by 10-4 mol/l cocaine. In the presence of atropine gallamine and pancuronium did not affect the release of3H-NE. This finding indicates that the effect of MR was mediated via presynaptic muscarinic receptors. Muscle relaxants and atropine inhibited these receptors and removed the tonic inhibitory effect of acetylcholine (ACh) released from the parasympathetic nerve endings on the release of NE from the sympathetic nerve. This was substantiated by the finding that in the present of cholinesterase inhibition, when the effect of endogenous ACh was amplified and thereby the cholinergic tone was dominant, the total release of3H-NE evoked by stimulation was much lower and muscle relaxants and atropine were much more effective to enhance3H-NE release. Gallamine and pancuronium also increased the force of contraction of the electrically stimulated atria. These findings indicate that the acceleration of the heart rate observed with gallamine and pancuronium in anesthetized man is due to increased release from, and not the inhibition of reuptake of NE by the sympathetic nerve endings of the right atrium.

Original languageEnglish
Pages (from-to)169-180
Number of pages12
JournalJournal of Neural Transmission
Volume76
Issue number3
DOIs
Publication statusPublished - Oct 1989

Fingerprint

Acetylcholine
Norepinephrine
Guinea Pigs
Gallamine Triethiodide
Pancuronium
Muscles
Atropine
Nerve Endings
Heart Atria
Cocaine
Presynaptic Receptors
Tubocurarine
Cholinesterases
Muscarinic Receptors
Cholinergic Agents
Heart Rate

Keywords

  • guinea pig atrium
  • muscarine receptors
  • muscle relaxants
  • Norepinephrine release
  • presynaptic inhibition

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Presynaptic effect of muscle relaxants on the release of3H-norepinephrine controlled by endogenous acetylcholine in guinea pig atrium. / Földes, F. F.; Kobayashi, O.; Kinjo, M.; Hársing, L.; Nagashima, H.; Duncalf, D.; Goldiner, P. L.; Vízi, E.

In: Journal of Neural Transmission, Vol. 76, No. 3, 10.1989, p. 169-180.

Research output: Contribution to journalArticle

Földes, F. F. ; Kobayashi, O. ; Kinjo, M. ; Hársing, L. ; Nagashima, H. ; Duncalf, D. ; Goldiner, P. L. ; Vízi, E. / Presynaptic effect of muscle relaxants on the release of3H-norepinephrine controlled by endogenous acetylcholine in guinea pig atrium. In: Journal of Neural Transmission. 1989 ; Vol. 76, No. 3. pp. 169-180.
@article{9780d402f30b44e480189d9f6b94dd6b,
title = "Presynaptic effect of muscle relaxants on the release of3H-norepinephrine controlled by endogenous acetylcholine in guinea pig atrium",
abstract = "The influence of nondepolarizing muscle relaxants (MR) on the resting and electrically evoked release of tritiated norepinephrine (3H-NE) was investigated, in the absence and presence of 10-4 mol/l cocaine, in the in vitro right atrium preparation of guinea pigs (g.p.) preloaded with3H-NE. In the absence of MR both resting and stimulated3H and3H-NE release remained relatively constant throughout the experiment and the ratios of the evoked release of3H during consecutive stimulation periods (i.e. S2/S1, S3/S2) were close to unity. None of the MR had any effect on resting3H release. Atropine (3× 10-7 mol/l), gallamine (7×10-5 mol/l), and pancuronium (2×10-6 mol/l), but not d-tubocurarine (5×10-6 mol/l) significantly increased stimulated release of3H-NE. The effect of MR on resting or evoked release of3H-NE was not influenced by 10-4 mol/l cocaine. In the presence of atropine gallamine and pancuronium did not affect the release of3H-NE. This finding indicates that the effect of MR was mediated via presynaptic muscarinic receptors. Muscle relaxants and atropine inhibited these receptors and removed the tonic inhibitory effect of acetylcholine (ACh) released from the parasympathetic nerve endings on the release of NE from the sympathetic nerve. This was substantiated by the finding that in the present of cholinesterase inhibition, when the effect of endogenous ACh was amplified and thereby the cholinergic tone was dominant, the total release of3H-NE evoked by stimulation was much lower and muscle relaxants and atropine were much more effective to enhance3H-NE release. Gallamine and pancuronium also increased the force of contraction of the electrically stimulated atria. These findings indicate that the acceleration of the heart rate observed with gallamine and pancuronium in anesthetized man is due to increased release from, and not the inhibition of reuptake of NE by the sympathetic nerve endings of the right atrium.",
keywords = "guinea pig atrium, muscarine receptors, muscle relaxants, Norepinephrine release, presynaptic inhibition",
author = "F{\"o}ldes, {F. F.} and O. Kobayashi and M. Kinjo and L. H{\'a}rsing and H. Nagashima and D. Duncalf and Goldiner, {P. L.} and E. V{\'i}zi",
year = "1989",
month = "10",
doi = "10.1007/BF01260502",
language = "English",
volume = "76",
pages = "169--180",
journal = "Journal of Neural Transmission",
issn = "0300-9564",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Presynaptic effect of muscle relaxants on the release of3H-norepinephrine controlled by endogenous acetylcholine in guinea pig atrium

AU - Földes, F. F.

AU - Kobayashi, O.

AU - Kinjo, M.

AU - Hársing, L.

AU - Nagashima, H.

AU - Duncalf, D.

AU - Goldiner, P. L.

AU - Vízi, E.

PY - 1989/10

Y1 - 1989/10

N2 - The influence of nondepolarizing muscle relaxants (MR) on the resting and electrically evoked release of tritiated norepinephrine (3H-NE) was investigated, in the absence and presence of 10-4 mol/l cocaine, in the in vitro right atrium preparation of guinea pigs (g.p.) preloaded with3H-NE. In the absence of MR both resting and stimulated3H and3H-NE release remained relatively constant throughout the experiment and the ratios of the evoked release of3H during consecutive stimulation periods (i.e. S2/S1, S3/S2) were close to unity. None of the MR had any effect on resting3H release. Atropine (3× 10-7 mol/l), gallamine (7×10-5 mol/l), and pancuronium (2×10-6 mol/l), but not d-tubocurarine (5×10-6 mol/l) significantly increased stimulated release of3H-NE. The effect of MR on resting or evoked release of3H-NE was not influenced by 10-4 mol/l cocaine. In the presence of atropine gallamine and pancuronium did not affect the release of3H-NE. This finding indicates that the effect of MR was mediated via presynaptic muscarinic receptors. Muscle relaxants and atropine inhibited these receptors and removed the tonic inhibitory effect of acetylcholine (ACh) released from the parasympathetic nerve endings on the release of NE from the sympathetic nerve. This was substantiated by the finding that in the present of cholinesterase inhibition, when the effect of endogenous ACh was amplified and thereby the cholinergic tone was dominant, the total release of3H-NE evoked by stimulation was much lower and muscle relaxants and atropine were much more effective to enhance3H-NE release. Gallamine and pancuronium also increased the force of contraction of the electrically stimulated atria. These findings indicate that the acceleration of the heart rate observed with gallamine and pancuronium in anesthetized man is due to increased release from, and not the inhibition of reuptake of NE by the sympathetic nerve endings of the right atrium.

AB - The influence of nondepolarizing muscle relaxants (MR) on the resting and electrically evoked release of tritiated norepinephrine (3H-NE) was investigated, in the absence and presence of 10-4 mol/l cocaine, in the in vitro right atrium preparation of guinea pigs (g.p.) preloaded with3H-NE. In the absence of MR both resting and stimulated3H and3H-NE release remained relatively constant throughout the experiment and the ratios of the evoked release of3H during consecutive stimulation periods (i.e. S2/S1, S3/S2) were close to unity. None of the MR had any effect on resting3H release. Atropine (3× 10-7 mol/l), gallamine (7×10-5 mol/l), and pancuronium (2×10-6 mol/l), but not d-tubocurarine (5×10-6 mol/l) significantly increased stimulated release of3H-NE. The effect of MR on resting or evoked release of3H-NE was not influenced by 10-4 mol/l cocaine. In the presence of atropine gallamine and pancuronium did not affect the release of3H-NE. This finding indicates that the effect of MR was mediated via presynaptic muscarinic receptors. Muscle relaxants and atropine inhibited these receptors and removed the tonic inhibitory effect of acetylcholine (ACh) released from the parasympathetic nerve endings on the release of NE from the sympathetic nerve. This was substantiated by the finding that in the present of cholinesterase inhibition, when the effect of endogenous ACh was amplified and thereby the cholinergic tone was dominant, the total release of3H-NE evoked by stimulation was much lower and muscle relaxants and atropine were much more effective to enhance3H-NE release. Gallamine and pancuronium also increased the force of contraction of the electrically stimulated atria. These findings indicate that the acceleration of the heart rate observed with gallamine and pancuronium in anesthetized man is due to increased release from, and not the inhibition of reuptake of NE by the sympathetic nerve endings of the right atrium.

KW - guinea pig atrium

KW - muscarine receptors

KW - muscle relaxants

KW - Norepinephrine release

KW - presynaptic inhibition

UR - http://www.scopus.com/inward/record.url?scp=0024520007&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024520007&partnerID=8YFLogxK

U2 - 10.1007/BF01260502

DO - 10.1007/BF01260502

M3 - Article

VL - 76

SP - 169

EP - 180

JO - Journal of Neural Transmission

JF - Journal of Neural Transmission

SN - 0300-9564

IS - 3

ER -