Preservation of trehalose-loaded red blood cells by lyophilization

Zsolt Török, Gyana R. Satpathy, Mitali Banerjee, Rachna Bali, Erika Little, Roberta Novaes, Hung Van Ly, Denis M. Dwyre, Azadeh Kheirolomoom, Fern Tablin, John H. Crowe, Nelly M. Tsvetkova

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A method for freeze-drying red blood cells (RBCs) while maintaining high viability has important implications in blood transfusion and clinical medicine. RBCs loaded with the disaccharide trehalose can be freeze-dried in a formulation of hydroxyethyl starch, human serum albumin, and trehalose to residual water contents between 2% and 4%. Rehydration of the freeze-dried erythrocytes resulted in about 55% survival, based on the percent hemolysis. The surviving cells can synthesize ATP and 2,3-DPG, have preserved morphology, and have low levels of methemoglobin. Biochemical analyses showed that the activities of superoxide dismutase and catalase in freeze-dried RBCs are very similar to those of fresh RBCs. Secondary structure of hemoglobin was similar to that of fresh hemoglobin. Trehalose loading is required to achieve the stabilization of hemoglobin. These data provide an important step towards a stable erythrocyte product, which can prove invaluable for transfusion and clinical applications.

Original languageEnglish
Pages (from-to)96-111
Number of pages16
JournalCell Preservation Technology
Volume3
Issue number2
DOIs
Publication statusPublished - Jul 27 2005

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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    Török, Z., Satpathy, G. R., Banerjee, M., Bali, R., Little, E., Novaes, R., Van Ly, H., Dwyre, D. M., Kheirolomoom, A., Tablin, F., Crowe, J. H., & Tsvetkova, N. M. (2005). Preservation of trehalose-loaded red blood cells by lyophilization. Cell Preservation Technology, 3(2), 96-111. https://doi.org/10.1089/cpt.2005.3.96