Preservation of algaenan and proteinaceous material during the oxic decay of Botryococcus braunii as revealed by pyrolysis-gas chromatography/mass spectrometry and 13C NMR spectroscopy

Reno T. Nguyen, H. Rodger Harvey, Xu Zang, Jasper D H Van Heemst, M. Hetényi, Patrick G. Hatcher

Research output: Contribution to journalArticle

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Abstract

Botryococcus braunii cells were grown until the late-stationary phase of growth and subsequently decomposed under oxic conditions for 201 days using a microbial consortium obtained from a freshwater lake. Degradation exhibited multi-G model kinetics, with a 'labile' fraction lost at a rate two to three times slower than those observed for the degradation of other previously studied phytoplankton, and a 'refractory' fraction lost even more slowly. Scanning electron microscopy of the 201-day detritus, as previously seen for the kerogen, indicates the preservation of cell wall material with loss of intracellular contents. Detrital samples analyzed by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and solid-state ramp-CPMAS 13C NMR, however, indicates the preservation of highly aliphatic material, algaenan, as well as 'intrinsically labile' proteinaceous components. These results further support the encapsulation hypothesis that proteins may be sterically protected from enzymatic attack via intimate associations with refractory, macromolecular organic matter.

Original languageEnglish
Pages (from-to)483-497
Number of pages15
JournalOrganic Geochemistry
Volume34
Issue number4
DOIs
Publication statusPublished - 2003

Fingerprint

algaenan
Gas chromatography
Refractory materials
pyrolysis
Nuclear magnetic resonance spectroscopy
Mass spectrometry
nuclear magnetic resonance
gas chromatography
Pyrolysis
mass spectrometry
spectroscopy
Kerogen
Degradation
degradation
encapsulation
Phytoplankton
kerogen
oxic conditions
Encapsulation
Biological materials

ASJC Scopus subject areas

  • Geochemistry and Petrology

Cite this

Preservation of algaenan and proteinaceous material during the oxic decay of Botryococcus braunii as revealed by pyrolysis-gas chromatography/mass spectrometry and 13C NMR spectroscopy. / Nguyen, Reno T.; Harvey, H. Rodger; Zang, Xu; Van Heemst, Jasper D H; Hetényi, M.; Hatcher, Patrick G.

In: Organic Geochemistry, Vol. 34, No. 4, 2003, p. 483-497.

Research output: Contribution to journalArticle

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AU - Hatcher, Patrick G.

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