Presequence-mediated intermembrane contact formation and lipid flow. A model membrane study

Z. Török, Rudy A. Demel, Johanna M. Leenhouts, Ben De Kruijff

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The ability of a synthetic peptide corresponding to the presequence of cytochrome c oxidase subunit IV from yeast to cause intermembrane contacts was investigated using monolayer techniques. The presequence inserted efficiently into the monolayer with a specificity for the mitochondrial cardiolipin. In the inserted form, the peptide strongly promoted the formation of close contacts with large unilamellar lipid vesicles present in the subphase, a property which was also specific for cardiolipin. The contacts formed were stable and tight and resulted in the flow of lipids from the vesicles to the monolayer. These results led to new suggestions on the involvement of intermembrane contact formation in mitochondrial protein import and membrane biogenesis.

Original languageEnglish
Pages (from-to)5589-5594
Number of pages6
JournalBiochemistry
Volume33
Issue number18
Publication statusPublished - 1994

Fingerprint

Unilamellar Liposomes
Cardiolipins
Monolayers
Membranes
Lipids
Peptides
Mitochondrial Proteins
Mitochondrial Membranes
Electron Transport Complex IV
Yeasts
Yeast

ASJC Scopus subject areas

  • Biochemistry

Cite this

Török, Z., Demel, R. A., Leenhouts, J. M., & De Kruijff, B. (1994). Presequence-mediated intermembrane contact formation and lipid flow. A model membrane study. Biochemistry, 33(18), 5589-5594.

Presequence-mediated intermembrane contact formation and lipid flow. A model membrane study. / Török, Z.; Demel, Rudy A.; Leenhouts, Johanna M.; De Kruijff, Ben.

In: Biochemistry, Vol. 33, No. 18, 1994, p. 5589-5594.

Research output: Contribution to journalArticle

Török, Z, Demel, RA, Leenhouts, JM & De Kruijff, B 1994, 'Presequence-mediated intermembrane contact formation and lipid flow. A model membrane study', Biochemistry, vol. 33, no. 18, pp. 5589-5594.
Török, Z. ; Demel, Rudy A. ; Leenhouts, Johanna M. ; De Kruijff, Ben. / Presequence-mediated intermembrane contact formation and lipid flow. A model membrane study. In: Biochemistry. 1994 ; Vol. 33, No. 18. pp. 5589-5594.
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