The aim of this review is to give a brief evaluation about the DNA-based diagnostic and typing methods for the most important foodborne pathogens, based on the available literature and on the authors' experiences with considerations for the adaptability of the present methods and for the need of faster and more specific tests. Main aspects of this evaluation are: cost and time effectiveness, sensitivity/specificity and internationally accepted standardization. Further important aspect is the compatibility to international databases so that these methods could also serve as widely available tools for molecular risk analysis. Some of the molecular methods reported here are already indispensable tools for diagnostic and typing studies in food safety. Through their data inputs for international databases, these molecular methods will be increasingly important in the future. However, the use of internationally standardized official (ISO) methods for systematic isolation and detection of representative pathogenic strains can not be replaced by the molecular detection methods yet.
|Translated title of the contribution||Present and future significance of molecular methods in microbial food safety examinations|
|Number of pages||12|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Jun 2014|
ASJC Scopus subject areas