Presence, uptake and localization of an immunoreactively interleukin 6 (IL-6)-like molecule in Tetrahymena pyriformis

L. Kőhidai, Péter Kovács, Eszter Lázár-Molnár, G. Csaba

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The unicellular Tetrahymena and its medium contain immunoreactively interleukin 6 (IL-6)-like molecules (hereinafter IL-6) in a measurable quantity in the 24 h-old cultures. This protozoan takes up exogenously supplied IL-6 very quickly, and this can be found in similar amounts in both the cells and the media after 1 h. After 24 h (48 h cultures), an equal amount of IL-6 is present in the control and IL-6-treated cells and their media. By 120 h, cells which have not had their medium changed retained the same quantity of IL-6 as the control; however less than half was found in IL-6-treated cells. In the medium of 120 h-old cultures, there was a reduction of IL-6 content relative to the 24 h content in the control; however, in the IL-6-treated cell culture medium, less than half of the level in the controls was found. Confocal microscopy demonstrated the localization of IL-6 in/on the oral apparatus and basal bodies, and the nuclear envelope also showed moderate labelling. IL-6 antibody binding was enhanced after IL-6 pretreatment (hormonal imprinting). The experiments call attention to the presence of an IL-6-like molecule and its uptake at a very low level of phylogeny. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)749-755
Number of pages7
JournalCell Biology International
Volume24
Issue number10
DOIs
Publication statusPublished - 2000

Fingerprint

Tetrahymena pyriformis
Interleukin-6
Basal Bodies
Tetrahymena
Nuclear Envelope
Phylogeny
Confocal Microscopy

Keywords

  • Hormonal imprinting
  • IL-6
  • Localization
  • Secretion
  • Tetrahymena
  • Uptake

ASJC Scopus subject areas

  • Cell Biology

Cite this

Presence, uptake and localization of an immunoreactively interleukin 6 (IL-6)-like molecule in Tetrahymena pyriformis. / Kőhidai, L.; Kovács, Péter; Lázár-Molnár, Eszter; Csaba, G.

In: Cell Biology International, Vol. 24, No. 10, 2000, p. 749-755.

Research output: Contribution to journalArticle

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