Presence of 'PSI free' LHCI and monomeric LHCII and subsequent effects on fluorescence characteristics in lincomycin treated maize

László Gáspár, E. Sárvári, Fermín Morales, Z. Szigeti

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cause of the strong non-photochemical fluorescence quenching was examined in maize (Zea mays L.) plants that were treated with lincomycin during the 72 h period of greening. They were deficient in core complexes but seemed to contain the full complement of antennae. The following results were obtained: (1) High FO could not be attributed to the dark reduction of Q A but to the presence of a high amount of not properly organized antenna complexes due to the inhibited synthesis of reaction centres. (2) On illumination fluorescence intensity dropped considerably below FO within 20 s, and reached a steady state still below FO. (3) Slowly relaxing part of non-photochemical quenching was significantly higher than in control plants. (4) De-epoxidation state was constant, and corresponded to the maximal value of the control. (5) Free Lhca1/4 dimers could be detected in all submembrane fractions, including the grana, obtained by digitonin fractionation. (6) Increase in the 679 and 700 nm fluorescence emissions could be attributed to the monomerisation of part of LHCII and to the presence of free Lhca2 or LHCII aggregates, respectively. (7) LHCII or PSII + LHCII and Lhca1/4 interaction may contribute to the increase of long-wavelength fluorescence in the granal fraction. We assume that the elevated fluorescence quenching of monomeric LHCII as well as the interaction between LHCII or PSII + LHCII and Lhca1/4 can be considered as an explanation for the extensive non-photochemical fluorescence quenching in lincomycin treated plants. The permanent presence of zeaxanthin may have contributed to the fast formation of quenching.

Original languageEnglish
Pages (from-to)1047-1057
Number of pages11
JournalPlanta
Volume223
Issue number5
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Lincomycin
lincomycin
Zea mays
Fluorescence
fluorescence
corn
antennae
digitonin
Digitonin
grana
Thylakoids
zeaxanthin
Lighting
lighting
wavelengths
fractionation
complement
synthesis

Keywords

  • Chlorophyll-proteins
  • Fluorescence emission
  • Fluorescence induction
  • Lincomycin
  • Zea

ASJC Scopus subject areas

  • Plant Science

Cite this

Presence of 'PSI free' LHCI and monomeric LHCII and subsequent effects on fluorescence characteristics in lincomycin treated maize. / Gáspár, László; Sárvári, E.; Morales, Fermín; Szigeti, Z.

In: Planta, Vol. 223, No. 5, 04.2006, p. 1047-1057.

Research output: Contribution to journalArticle

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AU - Szigeti, Z.

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AB - The cause of the strong non-photochemical fluorescence quenching was examined in maize (Zea mays L.) plants that were treated with lincomycin during the 72 h period of greening. They were deficient in core complexes but seemed to contain the full complement of antennae. The following results were obtained: (1) High FO could not be attributed to the dark reduction of Q A but to the presence of a high amount of not properly organized antenna complexes due to the inhibited synthesis of reaction centres. (2) On illumination fluorescence intensity dropped considerably below FO within 20 s, and reached a steady state still below FO. (3) Slowly relaxing part of non-photochemical quenching was significantly higher than in control plants. (4) De-epoxidation state was constant, and corresponded to the maximal value of the control. (5) Free Lhca1/4 dimers could be detected in all submembrane fractions, including the grana, obtained by digitonin fractionation. (6) Increase in the 679 and 700 nm fluorescence emissions could be attributed to the monomerisation of part of LHCII and to the presence of free Lhca2 or LHCII aggregates, respectively. (7) LHCII or PSII + LHCII and Lhca1/4 interaction may contribute to the increase of long-wavelength fluorescence in the granal fraction. We assume that the elevated fluorescence quenching of monomeric LHCII as well as the interaction between LHCII or PSII + LHCII and Lhca1/4 can be considered as an explanation for the extensive non-photochemical fluorescence quenching in lincomycin treated plants. The permanent presence of zeaxanthin may have contributed to the fast formation of quenching.

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