Presence of laminin fragments in cyst fluid from patients with autosomal dominant polycystic kidney disease (ADPKD): Role in proliferation of tubular epithelial cells

Martin J. Slade, Ruth B. Kirby, I. Pócsi, J. Kay Jones, Robert G. Price

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cyst fluid samples obtained from eight patients with autosomal dominant polycystic kidney disease (ADPKD) were analysed for the presence of the basement membrane component laminin and its breakdown products, using ELISA and immunoblotting techniques. Whole laminin was not detected, whereas laminin fragments of 270, 155, 87, 56, and 14 kDa were detected at a mean total value of ≃ 0.5 μ/ml. The laminin fragments were assessed for their effect on cultured normal and ADPKD epithelial cells. Both cell types showed accelerated growth under these conditions. These findings suggest that basement membrane degradative fragments present in cyst fluid may contribute to cystic epithelial cell proliferation and may therefore be important in the pathogenesis of ADPKD.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1401
Issue number2
DOIs
Publication statusPublished - Feb 4 1998

Fingerprint

Cyst Fluid
Autosomal Dominant Polycystic Kidney
Laminin
Epithelial Cells
Basement Membrane
Immunoblotting
Enzyme-Linked Immunosorbent Assay
Cell Proliferation
Growth

Keywords

  • Autosomal dominant polycystic kidney disease
  • Cyst fluid
  • Laminin fragment
  • Proliferation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

Presence of laminin fragments in cyst fluid from patients with autosomal dominant polycystic kidney disease (ADPKD) : Role in proliferation of tubular epithelial cells. / Slade, Martin J.; Kirby, Ruth B.; Pócsi, I.; Jones, J. Kay; Price, Robert G.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1401, No. 2, 04.02.1998, p. 203-210.

Research output: Contribution to journalArticle

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