Presence of intramucosal neuroglial cells in normal and aganglionic human colon

Kamran Badizadegan, Alyssa R. Thomas, Nandor Nagy, Dorothy Ndishabandi, Sarah A. Miller, Alessandro Alessandrini, Jaime Belkind-Gerson, Allan M. Goldstein

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The enteric nervous system (ENS) is composed of neural crest-derived neurons (also known as ganglion cells) the cell bodies of which are located in the submucosal and myenteric plexuses of the intestinal wall. Intramucosal ganglion cells are known to exist but are rare and often considered ectopic. Also derived from the neural crest are enteric glial cells that populate the ganglia and the associated nerves, as well as the lamina propria of the intestinal mucosa. In Hirschsprung disease (HSCR), ganglion cells are absent from the distal gut because of a failure of neural crest-derived progenitor cells to complete their rostrocaudal migration during embryogenesis. The fate of intramucosal glial cells in human HSCR is essentially unknown. We demonstrate a network of intramucosal cells that exhibit dendritic morphology typical of neurons and glial cells. These dendritic cells are present throughout the human gut and express Tuj1, S100, glial fibrillary acidic protein, CD56, synaptophysin, and calretinin, consistent with mixed or overlapping neuroglial differentiation. The cells are present in aganglionic colon from patients with HSCR, but with an altered immunophenotype. Coexpression of Tuj1 and HNK1 in this cell population supports a neural crest origin. These findings extend and challenge the current understanding of ENS microanatomy and suggest the existence of an intramucosal population of neural crest-derived cells, present in HSCR, with overlapping immunophenotype of neurons and glia. Intramucosal neuroglial cells have not been previously recognized, and their presence in HSCR poses new questions about ENS development and the pathobiology of HSCR that merit further investigation.

Original languageEnglish
Pages (from-to)G1002-G1012
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume307
Issue number10
DOIs
Publication statusPublished - Nov 15 2014

Keywords

  • Enteric glial cells
  • Enteric nervous system
  • Hirschsprung disease

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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    Badizadegan, K., Thomas, A. R., Nagy, N., Ndishabandi, D., Miller, S. A., Alessandrini, A., Belkind-Gerson, J., & Goldstein, A. M. (2014). Presence of intramucosal neuroglial cells in normal and aganglionic human colon. American Journal of Physiology - Gastrointestinal and Liver Physiology, 307(10), G1002-G1012. https://doi.org/10.1152/ajpgi.00164.2014