Presence of endogenous PACAP-38 ameliorated intestinal cold preservation tissue injury

A. Ferencz, G. Wéber, Z. Helyes, Hitoshi Hashimoto, Akemichi Baba, D. Reglodi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Cold preservation tissue injury remains an unsolved problem during small intestinal transplantation. Pituitary adenylate cyclase-activating polypeptide (PACAP) plays a central role in the intestinal physiology. The aim of our study was to compare the cold ischemic injury in wild-type and PACAP-38 deficient mice after small bowel cold storage. Cold ischemia was produced with small bowel preservation in a University of Wisconsin solution at 4°C in wild-type (n∈=∈35) mice for 1 h (GI), for 3 h (GII), and for 6 h (GIII); and in PACAP-38 deficient (n∈=∈35) mice for 1 h (GIV), for 3 h (GV), and for 6 h (GVI). Small bowel biopsies were collected after laparotomy (Control) and at the end of the ischemia periods. To determine oxidative stress parameters, malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) were measured. Tissue damage was analyzed by qualitative and quantitative methods on hematoxylin/eosin-stained sections. In PACAP-38 deficient animals, tissue lipid peroxidation was elevated. These changes were significant after 6 h (153.04∈±∈7.2) compared to sham-operated (110. 44∈±∈5.5) and compared to wild-type results (120.0∈±∈1.1 μmol/g, p∈

Original languageEnglish
Pages (from-to)428-434
Number of pages7
JournalJournal of Molecular Neuroscience
Volume42
Issue number3
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Tissue Preservation
Pituitary Adenylate Cyclase-Activating Polypeptide
Wounds and Injuries
Cold Ischemia
Hematoxylin
Eosine Yellowish-(YS)
Malondialdehyde
Laparotomy
Lipid Peroxidation
Superoxide Dismutase
Glutathione
Oxidative Stress
Ischemia
Transplantation
Biopsy

Keywords

  • Cold preservation
  • Oxidative stress
  • PACAP-38
  • Small bowel
  • Tissue injury

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Presence of endogenous PACAP-38 ameliorated intestinal cold preservation tissue injury. / Ferencz, A.; Wéber, G.; Helyes, Z.; Hashimoto, Hitoshi; Baba, Akemichi; Reglodi, D.

In: Journal of Molecular Neuroscience, Vol. 42, No. 3, 11.2010, p. 428-434.

Research output: Contribution to journalArticle

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