Presence of Cx37 and lack of desmin in smooth muscle cells are early markers for arteriogenesis

Wei Jun Cai, E. Kocsis, Dimitri Scholz, Xuegang Luo, Wolfgang Schaper, Jutta Schaper

Research output: Contribution to journalArticle

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Abstract

In search of early structural markers of arteriogenesis, we studied the expression of gap junction proteins as well as of contractile and cytoskeletal proteins in smooth muscle cells (SMCs) during coronary collateral vessel growth induced by chronic occlusion of the left circumflex artery (LCx) in the dog heart. We used confocal microscopy with antibodies against connexin37 (Cx37), α-smooth muscle actin (α-SM actin), calponin, desmin and vinculin. The quantitative confocal analysis of immunofluorescence intensity showed that (1) in normal vessels (NV), Cx37 was present in endothelium only, not in SMC. Calponin, α-SM actin, desmin and vinculin were evenly expressed in SMC. (2) In early growing V (EV) with minimal intima formation, α-SM actin, calponin and vinculin showed little change in SMC, but desmin was 3.3 times lower than in NV, and Cx37 was induced (NV 0 arbitrary units/μm2, EV 50.3). (3) In actively growing V (AV), α-SM actin, calponin and vinculin were 3-, 3.3- and 2.9-fold lower, respectively, in the neointima as compared to the media. However, Cx37 was 48.2 AU/μm2 in the media and 15.8 AU/μm2in the neointima. Desmin was almost absent in the neointima and 5-fold reduced in the media. SMC, strongly positive for α-SM actin and calponin, expressed Cx37. Our findings indicate that induction of Cx37 and reduction of desmin precede the phenotypic changes of SMCs, which are characterized by down-regulation of α-SM actin, calponin and vinculin, and the formation of a neointima. An altered expression of Cx37 and desmin, therefore, are early markers for arteriogenesis in dog heart.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume262
Issue number1-2
DOIs
Publication statusPublished - Jul 2004

Fingerprint

Desmin
Vinculin
Smooth Muscle Myocytes
Muscle
Actins
Neointima
Cells
Dogs
Contractile Proteins
Connexins
Cytoskeletal Proteins
Confocal microscopy
Confocal Microscopy
Endothelium
Fluorescent Antibody Technique
Smooth Muscle
calponin
Coronary Vessels
Down-Regulation
Arteries

Keywords

  • α-SM actin
  • arteriogenesis
  • Calponin
  • Connexin37
  • Desmin
  • Dog

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Presence of Cx37 and lack of desmin in smooth muscle cells are early markers for arteriogenesis. / Cai, Wei Jun; Kocsis, E.; Scholz, Dimitri; Luo, Xuegang; Schaper, Wolfgang; Schaper, Jutta.

In: Molecular and Cellular Biochemistry, Vol. 262, No. 1-2, 07.2004, p. 17-23.

Research output: Contribution to journalArticle

Cai, Wei Jun ; Kocsis, E. ; Scholz, Dimitri ; Luo, Xuegang ; Schaper, Wolfgang ; Schaper, Jutta. / Presence of Cx37 and lack of desmin in smooth muscle cells are early markers for arteriogenesis. In: Molecular and Cellular Biochemistry. 2004 ; Vol. 262, No. 1-2. pp. 17-23.
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