Presence of an enamel matrix protein derivative on human teeth following periodontal surgery.

Anton Sculean, Péter Windisch, Tibor Keglevich, Beata Fabi, Eva Lundgren, Peter Staale Lyngstadaas

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

An enamel matrix protein derivative (EMD) has been successfully employed to restore fully functional periodontal ligament, cementum, and alveolar bone in patients with severe attachment loss. When applied to denuded root surfaces, EMD is believed to assemble into a matrix that locally stimulates regenerative responses in the surrounding tissues. Since wound healing and induction of regeneration are biological processes typically running from weeks to months after treatment, the EMD matrix must be present on the root surface over a biologically significant time period to be effective. So far, no studies have been done in humans on the persistence of EMD on the treated root surface. The aim of the present study was to investigate the persistence of the EMD-based matrix on periodontally involved human teeth treated surgically with EMD. Sixteen patients with one intrabony defect each around teeth scheduled for extraction due to advanced periodontitis were treated by means of access flap surgery with and without application of EMD. At 1, 2, 3, and 4 weeks postsurgery, the teeth were removed and processed for histology and immunohistochemistry. The slides were stained using a rabbit anti-enamel-matrix-derivative (anti-EMD) antibody and goat-antirabbit secondary antibodies labeled with horseradish peroxidase (HRP). Immunohistochemical evaluation demonstrated the presence of EMD on all test root surfaces during the entire observation period of 4 weeks. No EMD was detected on any of the control roots. The results demonstrate for the first time in humans that EMD is present on treated root surfaces for up to 4 weeks following periodontal surgery.

Original languageEnglish
Pages (from-to)183-187
Number of pages5
JournalClinical Oral Investigations
Volume6
Issue number3
Publication statusPublished - Sep 2002

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Tooth
enamel matrix proteins
Biological Phenomena
Dental Cementum
Tooth Extraction
Periodontal Ligament
Antibodies
Periodontitis
Horseradish Peroxidase
Dental Enamel
Goats
Running
Wound Healing
Regeneration
Histology
Immunohistochemistry
Observation
Rabbits
Bone and Bones

ASJC Scopus subject areas

  • Dentistry(all)
  • Oral Surgery
  • Periodontics
  • Orthodontics

Cite this

Presence of an enamel matrix protein derivative on human teeth following periodontal surgery. / Sculean, Anton; Windisch, Péter; Keglevich, Tibor; Fabi, Beata; Lundgren, Eva; Lyngstadaas, Peter Staale.

In: Clinical Oral Investigations, Vol. 6, No. 3, 09.2002, p. 183-187.

Research output: Contribution to journalArticle

Sculean, Anton ; Windisch, Péter ; Keglevich, Tibor ; Fabi, Beata ; Lundgren, Eva ; Lyngstadaas, Peter Staale. / Presence of an enamel matrix protein derivative on human teeth following periodontal surgery. In: Clinical Oral Investigations. 2002 ; Vol. 6, No. 3. pp. 183-187.
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