Presence and distribution of biogenic amines (histamine, serotonin and epinephrine) in immunophenotyped human immune cells

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: In animal experiments many hormones were demonstrated in immune cells. However, very few data are at our disposal in the case of human immune cells. In an earlier experiment, ACTH, endorphin and T3 were studied and found in different subsets of human immune cells. Here, three biogenic amines (histamine, serotonin and epinephrine) were studied. Methods: Biogenic amine content of immunophenotyped human lymphocytes from 15 blood donors were investigated by multicolor flow cytometry using anti-biogenic amine antibodies. Monocytes and granulocytes separated by size and granularity were also studied. Results: Each biogenic amine could be detected in each subset of leukocytes, except epinephrine and serotonin in granulocytes. Activated T cells contained a higher amount of the amines, and CD19+B cells a higher amount of histamine, related to the whole lymphocyte population and to other subsets. Monocytes contained more histamine and epinephrine than lymphocytes and granulocytes contained twice as much histamine as monocytes and three times as much as lymphocytes. Conclusion: Human lymphocytes contain the three biogenic amine, similar to rat. However, while each amine was present in monocytes, in granulocytes serotonin and epinephrine were not demonstrated. The results call attention to the possible extrapolation of animal data to human lymphocytes and monocytes, but in the case of granulocytes, caution is needed. Taking into consideration earlier results, activated T cells appear to have an important role in the loss or production of hormones inside the immune system.

Original languageEnglish
Pages (from-to)530-534
Number of pages5
JournalInflammation Research
Volume57
Issue number11
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Biogenic Amines
Histamine
Epinephrine
Serotonin
Granulocytes
Monocytes
Lymphocytes
Amines
Hormones
Endorphins
T-Lymphocytes
Blood Donors
Adrenocorticotropic Hormone
Immune System
Flow Cytometry
Leukocytes
B-Lymphocytes
Antibodies
Population

Keywords

  • 5-HT
  • Biogenic amine
  • Epinephrine
  • Histamine
  • Human white blood cells
  • Immunophenotyping

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

@article{f1ec55b14af34e2ba9dc3ed79f7dd3c0,
title = "Presence and distribution of biogenic amines (histamine, serotonin and epinephrine) in immunophenotyped human immune cells",
abstract = "Objective: In animal experiments many hormones were demonstrated in immune cells. However, very few data are at our disposal in the case of human immune cells. In an earlier experiment, ACTH, endorphin and T3 were studied and found in different subsets of human immune cells. Here, three biogenic amines (histamine, serotonin and epinephrine) were studied. Methods: Biogenic amine content of immunophenotyped human lymphocytes from 15 blood donors were investigated by multicolor flow cytometry using anti-biogenic amine antibodies. Monocytes and granulocytes separated by size and granularity were also studied. Results: Each biogenic amine could be detected in each subset of leukocytes, except epinephrine and serotonin in granulocytes. Activated T cells contained a higher amount of the amines, and CD19+B cells a higher amount of histamine, related to the whole lymphocyte population and to other subsets. Monocytes contained more histamine and epinephrine than lymphocytes and granulocytes contained twice as much histamine as monocytes and three times as much as lymphocytes. Conclusion: Human lymphocytes contain the three biogenic amine, similar to rat. However, while each amine was present in monocytes, in granulocytes serotonin and epinephrine were not demonstrated. The results call attention to the possible extrapolation of animal data to human lymphocytes and monocytes, but in the case of granulocytes, caution is needed. Taking into consideration earlier results, activated T cells appear to have an important role in the loss or production of hormones inside the immune system.",
keywords = "5-HT, Biogenic amine, Epinephrine, Histamine, Human white blood cells, Immunophenotyping",
author = "E. P{\'a}llinger and G. Csaba",
year = "2008",
month = "11",
doi = "10.1007/s00011-008-8008-7",
language = "English",
volume = "57",
pages = "530--534",
journal = "Inflammation Research",
issn = "1023-3830",
publisher = "Birkhauser Verlag Basel",
number = "11",

}

TY - JOUR

T1 - Presence and distribution of biogenic amines (histamine, serotonin and epinephrine) in immunophenotyped human immune cells

AU - Pállinger, E.

AU - Csaba, G.

PY - 2008/11

Y1 - 2008/11

N2 - Objective: In animal experiments many hormones were demonstrated in immune cells. However, very few data are at our disposal in the case of human immune cells. In an earlier experiment, ACTH, endorphin and T3 were studied and found in different subsets of human immune cells. Here, three biogenic amines (histamine, serotonin and epinephrine) were studied. Methods: Biogenic amine content of immunophenotyped human lymphocytes from 15 blood donors were investigated by multicolor flow cytometry using anti-biogenic amine antibodies. Monocytes and granulocytes separated by size and granularity were also studied. Results: Each biogenic amine could be detected in each subset of leukocytes, except epinephrine and serotonin in granulocytes. Activated T cells contained a higher amount of the amines, and CD19+B cells a higher amount of histamine, related to the whole lymphocyte population and to other subsets. Monocytes contained more histamine and epinephrine than lymphocytes and granulocytes contained twice as much histamine as monocytes and three times as much as lymphocytes. Conclusion: Human lymphocytes contain the three biogenic amine, similar to rat. However, while each amine was present in monocytes, in granulocytes serotonin and epinephrine were not demonstrated. The results call attention to the possible extrapolation of animal data to human lymphocytes and monocytes, but in the case of granulocytes, caution is needed. Taking into consideration earlier results, activated T cells appear to have an important role in the loss or production of hormones inside the immune system.

AB - Objective: In animal experiments many hormones were demonstrated in immune cells. However, very few data are at our disposal in the case of human immune cells. In an earlier experiment, ACTH, endorphin and T3 were studied and found in different subsets of human immune cells. Here, three biogenic amines (histamine, serotonin and epinephrine) were studied. Methods: Biogenic amine content of immunophenotyped human lymphocytes from 15 blood donors were investigated by multicolor flow cytometry using anti-biogenic amine antibodies. Monocytes and granulocytes separated by size and granularity were also studied. Results: Each biogenic amine could be detected in each subset of leukocytes, except epinephrine and serotonin in granulocytes. Activated T cells contained a higher amount of the amines, and CD19+B cells a higher amount of histamine, related to the whole lymphocyte population and to other subsets. Monocytes contained more histamine and epinephrine than lymphocytes and granulocytes contained twice as much histamine as monocytes and three times as much as lymphocytes. Conclusion: Human lymphocytes contain the three biogenic amine, similar to rat. However, while each amine was present in monocytes, in granulocytes serotonin and epinephrine were not demonstrated. The results call attention to the possible extrapolation of animal data to human lymphocytes and monocytes, but in the case of granulocytes, caution is needed. Taking into consideration earlier results, activated T cells appear to have an important role in the loss or production of hormones inside the immune system.

KW - 5-HT

KW - Biogenic amine

KW - Epinephrine

KW - Histamine

KW - Human white blood cells

KW - Immunophenotyping

UR - http://www.scopus.com/inward/record.url?scp=58149107503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149107503&partnerID=8YFLogxK

U2 - 10.1007/s00011-008-8008-7

DO - 10.1007/s00011-008-8008-7

M3 - Article

C2 - 19109746

AN - SCOPUS:58149107503

VL - 57

SP - 530

EP - 534

JO - Inflammation Research

JF - Inflammation Research

SN - 1023-3830

IS - 11

ER -