Preparative-scale separation of polymyxins with an analytical high-performance liquid chromatography system by using displacement chromatography

H. Kalász, Csaba Horváth

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Polymyxin antibiotics were separated by using reversed-phase chromatography in the displacement mode with columns generally employed in analytical separations. By using an aqueous solution of dodecyloctyldimethylammonium chloride as the displacer more than 100 mg of commercial polymyxin B sulfate could be separated into its constituents on a 250 × 4.6 mm column packed with 5-μm octyl-silica. The results are compared to those obtained by elution chromatography and some of the main features of displacement chromatography are discussed.

Original languageEnglish
Pages (from-to)295-302
Number of pages8
JournalJournal of Chromatography A
Volume215
Issue numberC
DOIs
Publication statusPublished - Oct 23 1981

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Polymyxins
High performance liquid chromatography
Chromatography
High Pressure Liquid Chromatography
Polymyxin B
Reverse-Phase Chromatography
Silicon Dioxide
Chlorides
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

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AB - Polymyxin antibiotics were separated by using reversed-phase chromatography in the displacement mode with columns generally employed in analytical separations. By using an aqueous solution of dodecyloctyldimethylammonium chloride as the displacer more than 100 mg of commercial polymyxin B sulfate could be separated into its constituents on a 250 × 4.6 mm column packed with 5-μm octyl-silica. The results are compared to those obtained by elution chromatography and some of the main features of displacement chromatography are discussed.

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