Preparative scale purification of shidasterone, 2-deoxy-polypodine B and 9α,20-dihydroxyecdysone from Silene italica ssp. nemoralis

M. Báthori, Zita Pongrácz, Róbert Omacht, I. Máthé

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A suitable combination of preparative scale separation methods results in effective clean-up of the ecdysteroids of Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The isolation of minor ecdysteroids from the partially purified extract is based on the use of both droplet counter-current chromatography and low-pressure reversed-phase liquid chromatography. The purification is completed by preparative thin-layer chromatography and preparative high-performance liquid chromatography to obtain the minor ecdysteroids, such as 2-deoxy-20-hydroxyecdysone, shidasterone, 2-deoxy-polypodine B, makisterone C, and 9α,20-dihydroxyecdysone.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalJournal of Chromatographic Science
Volume42
Issue number5
Publication statusPublished - May 2004

Fingerprint

Silene
Ecdysteroids
Purification
Ecdysterone
Thin layer chromatography
Liquid chromatography
High performance liquid chromatography
Reverse-Phase Chromatography
Thin Layer Chromatography
Chromatography
High Pressure Liquid Chromatography
Pressure
2-deoxy-5beta,20-dihydroxyecdysone
shidasterone

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Preparative scale purification of shidasterone, 2-deoxy-polypodine B and 9α,20-dihydroxyecdysone from Silene italica ssp. nemoralis. / Báthori, M.; Pongrácz, Zita; Omacht, Róbert; Máthé, I.

In: Journal of Chromatographic Science, Vol. 42, No. 5, 05.2004, p. 275-279.

Research output: Contribution to journalArticle

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