Preparative on-line overpressure layer chromatography (OPLC)

A new separation technique for natural products

S. Nyiredy, C. A J Erdelmeier, K. Dallenbach-Toelke, K. Nyiredy-Mikita, O. Sticher

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Overpressure layer chromatography (oplc) is further developed for the online preparative isolation of natural compounds. Its use is demonstrated for the rapid isolation of one or two compounds in less than 1 h as well as for the isolation of more substances from plant extracts and/or prepurified fractions which require a few hours. Depending on the separation problem, sample sizes between 50 mg and 0.5 g may be applied to 20X20 cm silica plates. Principles of the method, practical considerations, and advantages of this techique over other available methodologies are discussed.

Original languageEnglish
Pages (from-to)885-891
Number of pages7
JournalJournal of Natural Products
Volume49
Issue number5
Publication statusPublished - 1986

Fingerprint

Plant Extracts
Chromatography
Biological Products
Silicon Dioxide
Sample Size
chromatography
plant extracts
silica
methodology
sampling

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Nyiredy, S., Erdelmeier, C. A. J., Dallenbach-Toelke, K., Nyiredy-Mikita, K., & Sticher, O. (1986). Preparative on-line overpressure layer chromatography (OPLC): A new separation technique for natural products. Journal of Natural Products, 49(5), 885-891.

Preparative on-line overpressure layer chromatography (OPLC) : A new separation technique for natural products. / Nyiredy, S.; Erdelmeier, C. A J; Dallenbach-Toelke, K.; Nyiredy-Mikita, K.; Sticher, O.

In: Journal of Natural Products, Vol. 49, No. 5, 1986, p. 885-891.

Research output: Contribution to journalArticle

Nyiredy, S, Erdelmeier, CAJ, Dallenbach-Toelke, K, Nyiredy-Mikita, K & Sticher, O 1986, 'Preparative on-line overpressure layer chromatography (OPLC): A new separation technique for natural products', Journal of Natural Products, vol. 49, no. 5, pp. 885-891.
Nyiredy, S. ; Erdelmeier, C. A J ; Dallenbach-Toelke, K. ; Nyiredy-Mikita, K. ; Sticher, O. / Preparative on-line overpressure layer chromatography (OPLC) : A new separation technique for natural products. In: Journal of Natural Products. 1986 ; Vol. 49, No. 5. pp. 885-891.
@article{9c1cddc5f48947c29616bb447d6667d1,
title = "Preparative on-line overpressure layer chromatography (OPLC): A new separation technique for natural products",
abstract = "Overpressure layer chromatography (oplc) is further developed for the online preparative isolation of natural compounds. Its use is demonstrated for the rapid isolation of one or two compounds in less than 1 h as well as for the isolation of more substances from plant extracts and/or prepurified fractions which require a few hours. Depending on the separation problem, sample sizes between 50 mg and 0.5 g may be applied to 20X20 cm silica plates. Principles of the method, practical considerations, and advantages of this techique over other available methodologies are discussed.",
author = "S. Nyiredy and Erdelmeier, {C. A J} and K. Dallenbach-Toelke and K. Nyiredy-Mikita and O. Sticher",
year = "1986",
language = "English",
volume = "49",
pages = "885--891",
journal = "Journal of Natural Products",
issn = "0163-3864",
publisher = "American Chemical Society",
number = "5",

}

TY - JOUR

T1 - Preparative on-line overpressure layer chromatography (OPLC)

T2 - A new separation technique for natural products

AU - Nyiredy, S.

AU - Erdelmeier, C. A J

AU - Dallenbach-Toelke, K.

AU - Nyiredy-Mikita, K.

AU - Sticher, O.

PY - 1986

Y1 - 1986

N2 - Overpressure layer chromatography (oplc) is further developed for the online preparative isolation of natural compounds. Its use is demonstrated for the rapid isolation of one or two compounds in less than 1 h as well as for the isolation of more substances from plant extracts and/or prepurified fractions which require a few hours. Depending on the separation problem, sample sizes between 50 mg and 0.5 g may be applied to 20X20 cm silica plates. Principles of the method, practical considerations, and advantages of this techique over other available methodologies are discussed.

AB - Overpressure layer chromatography (oplc) is further developed for the online preparative isolation of natural compounds. Its use is demonstrated for the rapid isolation of one or two compounds in less than 1 h as well as for the isolation of more substances from plant extracts and/or prepurified fractions which require a few hours. Depending on the separation problem, sample sizes between 50 mg and 0.5 g may be applied to 20X20 cm silica plates. Principles of the method, practical considerations, and advantages of this techique over other available methodologies are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0040549174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0040549174&partnerID=8YFLogxK

M3 - Article

VL - 49

SP - 885

EP - 891

JO - Journal of Natural Products

JF - Journal of Natural Products

SN - 0163-3864

IS - 5

ER -