Preparative medium pressure liquid chromatographic (MPLC) and semipreparative HPLC separation of furocoumarin isomers

G. C. Zogg, Sz Nyiredy, O. Sticher

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Abstract

Preparative medium pressure liquid chromatography and semi-preparative high performance liquid chromatography on silica were employed for the isolation of eight furocoumarin isomers (iso-bergapten, angelicin, psoralen, bergapten, pimpinellin, sphondin, xanthotoxin and isopimpinellin). The conditions were transposed from analytical on-line overpressured layer chromatography and analytical high performance liquid chromatography, respectively. The retention data of the analytical on-line overpressured layer chromatographic separation and the preparative medium pressure liquid chromatographic isolation show a linear relationship. Using 15 μm TLC silica 60 as stationary phase a dry-packing procedure aas developed for labomatic medium pressure liquid chromatographic glass columns. The efficiency of the preparative separation techniques was demonstrated by the isolation of the investigated furocoumarins from Heraclei mantegazziani radix and from Pimpinellae radix (Balkan quality).

Original languageEnglish
Pages (from-to)591-595
Number of pages5
JournalChromatographia
Volume27
Issue number11-12
DOIs
Publication statusPublished - Jun 1 1989

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Keywords

  • Column liquid chromatography
  • Furocoumarin isomers
  • Overpressured layer chromatography
  • Preparative separation of plant extracts

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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